Fig 1: Erk2 regulates the output of miR-549a via XPO5. (A) Cytosolic and nuclear protein were prepared and analyzed for XPO5 expression in 786-O and 786-O-SR by Western blot. (B) Western blot analysis of Erk1/2 expression in 786-O and 786-O-SR and the detection of overexpression efficiency of Erk2 in 786-O. (C) RT-PCR and gel electrophoresis of PCR products analysis of pre-miR-549a expression in 786-O/786-O-SR cells. (D) Cytosolic and nuclear XPO5 expression in 786-O and Erk2 overexpressing 786-O by Western blotting. (E,F) RT-PCR and gel electrophoresis of PCR products analysis of pre-miR-549a expression in 786-O/786-O-SR exosomes (E) and HUVECs treated with exosomes (F). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 according to two-tailed Student’s t-test.