FTO (D6Z8W) Rabbit mAb 31687T from Cell Signaling Technology

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Cell communication and signaling : CCS
November 02 2023

Fig 1: The FAK inhibitor defactinib (VS6063) can suppress NSCLC metastasis induced by overexpression of FTO in vivo. a GSEA based on the TCGA dataset showed that high expression of FTO was associated with focal adhesion. ES, enrichment score. NES, normalized enrichment score. FDR, false discovery rate. b Western blot analysis showed that FTO can affect FAK signaling. c A flowchart of the NSCLC cell in vivo metastasis model. A549 cells overexpressing FTO or empty vector (1.8 × 106 cells/mouse) were injected i.v. into BALB/c nude mice (n = 5 mice per group). VS6063 or DMSO was administered i.g. as described in the Methods beginning in week 5. d The relative mRNA and protein expression levels of FTO in the A549 cells used to establish the in vivo metastasis model. e Photographs of lung metastatic nodules that developed in mice after injection of FTO-overexpressing or control vector A549 cells and administration of DMSO or VS6063. The red arrowheads indicate metastatic nodules that developed in the lungs. f Comparison of the number of lung metastatic nodules among the three groups. g H&E staining was carried out to evaluate lung micrometastatic foci. Representative histological images of micrometastatic foci in the three groups. The red arrowheads denote micrometastatic foci. Scale bar, 100 μm. Data information: Data are shown as the mean ± SDs. In all relevant panels, *P < 0.05; **P < 0.01; ***P < 0.001
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Frontiers in oncology
January 01 2022

Fig 2: FTO Promotes Cell Survival through PDGFRB/ERK Signaling Axis. (A–C) GSEA bar plot showing gene sets enriched in high FTO expression group (A). GSEA enrichment plots of MAPK (B) and ERK (C) associated gene sets. NES, normalized enrichment score; FDR, false discovery rate. (D) OCI-AML3 cells were transfected with the HA-FTO plasmids for 48 h, following treatment with the ERK cascade inhibitor U0126, the JNK cascade inhibitor SP600125, and the p38 cascade inhibitor SB203580 at 5 μM for 24 h, respectively. CCK-8 analysis of cell proliferation in the treated cells. (E) Western blot analysis of FTO, ERK, p-ERK, JNK, and p-JNK protein level in the FTO-silenced OCI-AML3 cells. (F) The FTO-silenced OCI-AML3 cells were transfected with the HA-FTO plasmids. Western blot analysis of FTO, ERK, and p-ERK protein level in the treated cells. (G) Western blot analysis of FTO, ERK, and p-ERK protein level in OCI-AML2 cells transfected with the HA-FTO plasmids. β-actin was used as the internal control. The bar graphs showed the relative level of protein. (H) qRT-PCR analysis of the FTO, PDGFRB, IGF1R, VEGFR, and EGFR mRNA level in the FTO-silenced OCI-AML3 cells. (I) Prediction score of m6A distribution in PDGFRB mRNA sequence using SRAMP. (J) qRT-PCR analysis of the PDGFRB mRNA level in OCI-AML2 and OCI-AML3 cells. (K) qRT-PCR analysis of the PDGFRB mRNA level in primary AML blasts without (n=21) and with (n=15) NPM1 mutations. (L) Western blot analysis of the FTO, ERK, p-ERK, and PDGFRB protein level in the FTO-silenced OCI-AML3 cells after PDGFRB knockdown. β-actin was used as the internal control. (M) CCK-8 analysis of cell proliferation in the NPM1-mA-silenced OCI-AML3 cells transfected with the HA-FTO plasmids for 48 h, following the treatment with 5 μM U0126 for 24 h. Data were presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001; N.S. indicated not significant.
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Frontiers in oncology
January 01 2022

Fig 3: NPM1-mA Upregulates FTO Expression to Reduce the m6A Level through Impeding the Proteasome Pathway. (A, C) Western blot analysis of the FTO, NPM1-mA, and NPM1-wt protein level in the NPM1-mA-silenced OCI-AML3, NPM1-wt-enforced OCI-AML2, and NPM1-mA-enforced OCI-AML2 cells. The bar graph showed the relative level of protein. (B, D) qRT-PCR analysis of the FTO, NPM1-mA, and NPM1-wt mRNA level in the NPM1-mA-silenced OCI-AML3, NPM1-wt-enforced OCI-AML2, and NPM1-mA-enforced OCI-AML2 cells. (E) OCI-AML3 cells were treated with the ubiquitin-proteasome pathway inhibitor MG132 (20 μM), the autophagic-lysosomal pathway inhibitor CQ (20 μM), and activator rapamycin (20 μM) for 8 h. The bar graph showed the relative level of FTO protein. (F) Western blot analysis of the FTO protein level in the NPM1-silenced OCI-AML3 cells following treatment with MG132 (20 μM) for 8h. The bar graph showed the relative level of FTO protein. (G) Western blot analysis of the FTO and NPM1-mA protein level in the NPM1-mA-silenced OCI-AML3 cells following treatment with the protein synthesis inhibitor CHX (100 μg/mL) for the indicated time. The line graph showed the degradation rate of FTO protein. (H) Ubiquitination analysis of the ubiquitinated FTO level in the NPM1-mA-silenced OCI-AML3 cells. β-actin was used as the internal control. (I, J) m6A dot blot analysis of the global m6A abundance in the NPM1-mA-silenced OCI-AML3, NPM1-wt-enforced OCI-AML2, and NPM1-mA-enforced OCI-AML2 cells. Methylene blue staining served as a loading control. (K) Western blot analysis of the FTO protein level in the NPM1-mA-silenced OCI-AML3 cells transfected with the HA-FTO plasmids. The bar graph showed the relative level of FTO protein. β-actin was used as the internal control. (L) m6A dot blot analysis of the global m6A abundance in the NPM1-mA-silenced OCI-AML3 cells transfected with the HA-FTO plasmids. Methylene blue staining served as a loading control. Data were presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001; N.S. indicated not significant.
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Frontiers in Oncology
July 01 2021

Fig 4: Construction of weight co-expression modules of gastric cancer with FTO expression. (A) The clustering was based on the expression data from TGCA. (B) The left figure showed the correlation of various soft-thresholding power and scale independence. The right figure showed the effect of soft threshold power values on mean connectivity. (C) Similar gene modules were merged according to the MEDissThres 0.25 (Red line) by calculating eigengenes of each module. (D) A hierarchical clustering dendrogram was conducted by the Dynamic Tree Cutting method. The first color band indicates the modules detected by dynamic tree cut. The second color band indicates the modules after merging similar modules.
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Frontiers in Oncology
July 01 2021

Fig 5: FTO promoted GC cell migration and invasion. (A) The relative mRNA and protein expression of FTO in different gastric cancer cell lines were detected by qRT-PCR and Western blot. (B) The knockdown efficiency of FTO in BGC823 and MGC803 cells was detected by qRT-PCR. (C, D) The migration and invasion ability of BGC823 and MGC803 cells after transfected with siNC or FTO siRNAs was examined by transwell assay (original magnification, 100×). The columns on the right are quantified by counting 3 fields, and presented as the mean ± standard deviation. (E, F) The proliferation ability of BGC823 and MGC803 cells after transfected with siNC or FTO siRNAs was examined by MTT and colony-formation assay. The columns are presented as the mean ± standard deviation. Data are presented as the mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001. 18S was used as an internal control for all qRT-PCR experiments. GAPDH was used as an internal control for all western blot assays.

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Cell Signaling Technology for
FTO (D6Z8W) Rabbit mAb

Cell Signaling Technology's FTO (D6Z8W) Rabbit mAb is a Rabbit monoclonal antibody. The FTO (D6Z8W) Rabbit mAb Antibody was generated using FTO, and FTO alpha-ketoglutarate dependent dioxygenase as the antigen. It reacts with Homo Sapiens, and Human. This antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot.

Product Specs
ItemFTO (D6Z8W) Rabbit mAb
CompanyCell Signaling Technology
Price $153.00 Inquire
Catalog Number31687T
Quantity20 µl
ApplicationsW, IP
ReactivityH
HostRabbit
TargetFTO
ClonalityMonoclonal
NCBI Full Gene NameFTO alpha-ketoglutarate dependent dioxygenase
NCBI Gene AliasesALKBH9, BMIQ14, GDFD, IFEX9

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(1) Elevated m6A RNA modifications associate with immune dysregulation and cancer in people with HIV-1BMC CancerMarch 21, 2026Tarun Mishra, Shraddha Tripathi, Jack T. Stapleton, Li WuInvitrogen, PA5-89122), YTHDF3 (1:5000, CST, 80303), ALKBH5 (1:5000, Sigma, HPA007196), and FTO (1:3000, CST, 31687). Membranes were then incubated with HRP-conjugated secondary antibodies for 1 h and visualized using

(2) Directed evolution of an m6A eraser for site-selective epitranscriptome editingResearch SquareFebruary 23, 2026Chuan-Hui Zhou, Chuan-Hui WangGAPDH, and HRP-conjugated anti-rabbit secondary antibody were purchased from Cell Signaling Technology (31687T, 2118T, 7074P2). Protein expression and purification. Recombinant human FTO truncated at the N-terminal

(3) RBM15B recognizes H3K79me2 to guide selective m6A-modification of mRNA and enhance oncoprotein translation in MLL-r leukemiaThe EMBO JournalFebruary 2, 2026Tian-Qi Chen, Yu-Meng Sun, Shun-Xin Zhu, Xiao-Tong Chen, Ke-Jia Pu, Heng-Jing Huang, Qi Pan, Jun-Yi Lian, Wei Huang, Ke Fang, et al.,Novus Biologicals,NBP1-81392 Mouse anti-WTAP,Proteintech,60188-1-Ig Rabbit anti-FTO,Cell Signaling Technology,31687 Rabbit anti-DOT1L,Cell Signaling Technology,77087 Rabbit anti-MLL1,Cell Signaling Technology,14689 Rabbit

(4) Mechanism of LncRNA NORAD regulating ferroptosis in endometrial cancer cells by modifying GPX4 through FTO-mediated m6A methylationCell Mol Life SciOctober 25, 2025Jieqi Ke, Ting Gao, Zhen Shen, Min Li, Tianjiao Zhang, Dabao Wulysis buffer containing protease inhibitors and incubated with the antibodies rabbit anti-FTO (1:50, #31687, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-YTHDF2 (1:30, ab220163, Abcam), IgG (diluted

(5) M6A Modified miR‐31‐5p Suppresses M1 Macrophage Polarization and Autoimmune Dry Eye by Targeting P2RX7Advanced ScienceMarch 11, 2025Lu Zhao, Xuejia Li, Min Gao, Lin Liu, Binyun Ma, Xun Liu, Jiachen Zhang, Ruoxuan Liu, Bei Du, Ruihua Wei, et al.#13809), DGCR8 (1:1000, Abcam, UK, cat. ab191875), FTO (1:1000, Cell Signaling Technology, USA, cat. # 31687) or β‐actin (1:2000, ZSGB‐BIO, China, cat. TA‐09) and detected using Tanon 4800 Multispectral Imaging

(6) MAT2B regulates the protein level of MAT2A to preserve RNA N6-methyladenosineCell death & diseaseOctober 1, 2024Wan X, Zeng W, Fan H, Wang C, Han S, Sun Z, Tang M, Shao J, Liu Y, Fang Y, et al.(Cell Signaling Technology, Cat. #51104); Rabbit polyclonal anti-FTO (Cell Signaling Technology, Cat. #31687); Rabbit polyclonal anti-ALKBH5 (Cell Signaling Technology, Cat. #80283); Rabbit polyclonal anti-MTHFD1

(7) Long non-coding RNA GATA6-AS1 is mediated by N6-methyladenosine methylation and inhibits the proliferation and metastasis of gastric cancerWorld Journal of Gastrointestinal OncologyMarch 15, 2024Jun-Jie Shen, Min-Chang Li, Shao-Qi Tian, Wen-Ming Chendetected using a ChemiDo imaging system (Bio-Rad). The following antibodies were used: anti-FTO (1:5000, 31687, Cell Signaling Technology, United States), anti-YTH domain-containing family protein 1 (1: 5000, 86463,

(8) The m6A demethylase fat mass and obesity-associated protein mitigates pyroptosis and inflammation in doxorubicin-induced heart failure via the toll-like receptor 4/NF-κB pathwayCardiovascular Diagnosis and TherapyFebruary 15, 2024Weiling Tu, Xiao Huang, Songtao Liu, Yuliang Zhan, Xinyong Cai, Liang Shaodetail, the cells were submerged in the RIP lysis buffer and reacted with antibodies against FTO [1:50, #31687, Cell Signaling Technologies (CST), Beverly, MA, USA], YTH N6-methyladenosine RNA binding protein 1

(9) The involvement of RNA N6‐methyladenosine and histone methylation modification in decidualization and endometriosis‐associated infertilityClinical and Translational MedicineFebruary 12, 2024Xiang Lin, Yongdong Dai, Weijia Gu, Yi Zhang, Feng Zhuo, Fanxuan Zhao, Xiaoying Jin, Chao Li, Dong Huang, Xiaomei Tong, et al.applied,Abcam,UK YTHDF2,80014,1:1000,1:100,Not applied,CST,USA IGFBP1,sc‐55474,1:800,1:100,Not applied,SANTA,USA FTO,31687,1:1000,Not applied,Not applied,CST,USA METTL3,86132,1:1000,Not applied,Not applied,CST,USA WTAP,56501,1:1000,Not

(10) FTO facilitates cancer metastasis by modifying the m(6)A level of FAP to induce integrin/FAK signaling in non-small cell lung cancerCell communication and signaling : CCSNovember 2, 2023Gao L, Wang A, Chen Y, Cai X, Li Y, Zhao J, Zhang Y, Zhang W, Zhu J, Zeng Y, et al.according to our previous research [32]. The following antibodies were utilized in this study: anti-FTO (#31687), anti-FAP (#66562), anti-Snail (#3895), anti-Slug (#9585), anti-N-cadherin (#13116), anti-FAK (#13009),

(11) Characterization of circSCL38A1 as a novel oncogene in bladder cancer via targeting ILF3/TGF-β2 signaling axisCell death & diseaseJanuary 25, 2023Li P, Mi Q, Yan S, Xie Y, Cui Z, Zhang S, Wang Y, Gao H, Wang Y, Li J, et al.include Vimentin (5741 S, CST), Snail (3879 S, CST), ILF3 (ab133354, Abcam), METTL3 (96391, CST), FTO (31687 S, CST), HA-tag (3724 S, CST), TGF beta 2 (36495, Abcam), N-cadherin (13116 S, CST), CD9 (13403, CST),

(12) Cancer-associated fibroblasts promote migration and invasion of non-small cell lung cancer cells via METTL3-mediated RAC3 m(6)A modificationInternational journal of biological sciencesJanuary 1, 2023Chen M, Zhang Q, Zheng S, Guo X, Cao L, Ren Y, Qian Y, Wang M, Wu X, Xu K.antibody were purchased from R&D Systems (Minneapolis, MN). Antibodies against METTL3 (#86132), FTO (#31687), AKT (#4685), p-AKT (#4060), and p65 (#8242) (in 1:1000 dilution) were purchased from Cell Signaling Technology

(13) Hydrogen sulfide mitigates skeletal muscle mitophagy-led tissue remodeling via epigenetic regulation of the gene writer and eraser functionPhysiological reportsAugust 1, 2022Singh M, Pushpakumar S, Zheng Y, Homme RP, Smolenkova I, Mokshagundam SPL, Tyagi SC.grade. The antibodies for ten‐eleven translocate 2 (TET2, #36449), fat obese associated protein (FTO, #31687), di‐methyl‐histone (Lys9) (H3K9, #4658), and matrix metalloproteinases‐13 (MMP13, #69926) were ordered

(14) RNA-Seq data of ALKBH5 and FTO double knockout HEK293T human cellsData in BriefJune 1, 2022Egor A. Smolin, Andrey I. Buyan, Dmitry N. Lyabin, Ivan V. Kulakovskiy, Irina A. Eliseevaby limiting dilution and screened by immunoblotting with anti-ALKBH5 (#ab195377, Abcam), anti-FTO (#31687, CST), and anti-ACTB (loading control, #ab8227, Abcam) antibodies as was described previously [10].

(15) Mutant NPM1-Regulated FTO-Mediated m(6)A Demethylation Promotes Leukemic Cell Survival via PDGFRB/ERK Signaling AxisFrontiers in oncologyJanuary 1, 2022Xiao Q, Lei L, Ren J, Peng M, Jing Y, Jiang X, Huang J, Tao Y, Lin C, Yang J, et al.beads (Bimake, #B23201) which were coated with an anti-FTO antibody (1:50, Cell Signaling Technology, #31687) at 4°C overnight. The protein A/G beads combined with immunocomplexes were washed with PBST three times

(16) ALKBH5-mediated m⁶A demethylation of KCNK15-AS1 inhibits pancreatic cancer progression via regulating KCNK15 and PTEN/AKT signalingCell death & diseaseDecember 1, 2021He Y, Yue H, Cheng Y, Ding Z, Xu Z, Lv C, Wang Z, Wang J, Yin C, Hao H, et al.(ab195352, Abcam, 1/50), METTL14 (26158-1-AP, Proteintech, 1/50), WTAP (ab195380, Abcam, 1/40), FTO (#31687, Cell signaling technology, 1/50), ALKBH5 (16837-1-AP, Proteintech, 1/50), MDM2 (ab226939, Abcam, 1/100),

(17) N6-Methyladenosine Methyltransferase METTL14-Mediated Autophagy in Malignant Development of Oral Squamous Cell CarcinomaFrontiers in OncologyNovember 24, 2021Fang Wang, Yue Zhu, Hongshi Cai, Jianfeng Liang, Wenjin Wang, Yan Liao, Yadong Zhang, Cheng Wang, Jinsong HouPrimary antibodies included anti-mTOR (#2983), anti-METTL3 (#86132), anti-WTAP (#41934), anti-FTO (#31687), anti-ALKBH5 (#80283), anti-LC3B (#4599), anti-eIF4G1 (#2858) (1:1 000, Cell Signaling Technology,

(18) METTL3 regulates hippocampal gene transcription via N6-methyladenosine methylation in sevoflurane-induced postoperative cognitive dysfunction mouseAgingOctober 5, 2021He B, Wang J.were incubated with 1 μg antibodies of m6A (56593S, CST, Beverly, MA, USA), METTL3 (86132, CST), FTO (31687, CST), ALKBH5 (16837-1-AP, Proteintech, Rosemont, IL, USA), and IGF2BP1 (22803-1-AP, Proteintech) for

(19) Dynamic Patterns of N6-Methyladenosine Profiles of Messenger RNA Correlated with the Cardiomyocyte Regenerability during the Early Heart Development in MiceOxidative Medicine and Cellular LongevityAugust 6, 2021Yuhui Yang, Siman Shen, Yin Cai, Kejun Zeng, Keyu Liu, Simeng Li, Lanfen Zeng, Linming Chen, Jing Tang, Zhe Hu, et al.MA, USA), anti-YTHDF1 (ab252346, Abcam, MA, USA), anti-IGF2BP3 (ab179807, Abcam, MA, USA), anti-FTO (31687S, CST, MA, USA), and anti-GAPDH (ab8227, Abcam, MA, USA). The secondary antibodies were goat polyclonal

(20) N6-Methyladenosine RNA Demethylase FTO Promotes Gastric Cancer Metastasis by Down-Regulating the m6A Methylation of ITGB1Frontiers in OncologyJuly 1, 2021Duo Wang et al.heat-inactivated FBS. They were incubated at 37°C in saturated humidity with 5% CO2. Rabbit anti-FTO (#31687), anti-integrin β1 (#9699S), anti-GAPDH (#2118) and anti-LAMC1 (#4577) were obtained from Cell Signaling Technology

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Cell communication and signaling : CCS

Frontiers in oncology

Frontiers in oncology

Frontiers in Oncology

Frontiers in Oncology

FTO (D6Z8W) Rabbit mAb from Cell Signaling Technology

Cell Signaling Technology

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FTO (D6Z8W) Rabbit mAb from Cell Signaling Technology

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