Fig 1: Enriched pathways and genes in the cholangiocytes of BA. (A) Flowchart of isolating BA epithelial cells from the merged groups. (B) t-SNE visualization of hep, rep, and cho in BA. (C) Violin plots showing the expression levels of the marker genes of hep, rep, and cho in BA. (D) Heatmap displaying enriched pathways of cholangiocytes in BA by GSVA analysis. (E) Violin plots displayed the EMT score among the 3 types of epithelial cells in BA. (F) Heatmap displaying the average expression level of EMT markers, fibrotic markers, inflammatory genes, and m6A readers in BA hep, rep, and cho. (G) Representative immunohistochemistry sections of CDH2, smad2/3, FGFR2, PDGFA, CXCL6, and IGF2BP2 in a TMA. Dashed circles in PDGFA were recognized as cholangiocytes. H. Representative immunofluorescence staining of CDH2, Smad2/3, FGFR2, PDGFA, CXCL6, and IGF2BP2 in TMA of BA group. I. Box plots showing the expression levels of the indicated genes in TMA, colored by different groups. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. Abbreviations: BA, biliary atresia; CHO, cholangiocytes; CS, cholestasis; EMT, epithelial-mesenchymal transition; HEP, hepatocytes; NC, normal control; REP, reprogrammed cells; TMA, tissue microarray.