Fig 1: miR-142-3p activates NHEJ by targeting FOXO4, thereby promoting DNA damage repair. (A) Advanced volcano plot of RNA-seq comparing miR-142-3p over-expression cells with LV control cells. (B, C) KEGG Enrichment Bubble Plot and KEGG Enrichment Scatter Plot of RNA-seq comparing miR-142-3p over-expression cells with LV control cells. (D) GO Enrichment Scatter Plot of RNA-seq comparing miR-142-3p overexpression cells with LV control cells. (E) Western blot analysis of DNA damage response proteins (DNA-PKcs, Ku80, RAD50, MRE11, NBS1, XRCC1, LIG3) in SW480 and SW620 cells under different treatments (NC, LV, si-FOXO4, miR-142-3p, miR-142-3p+FOXO4). β-actin was used as the loading control. (F) Western blot analysis of γH2AX in SW480 and SW620 cells treated with 6 Gy of radiation. β-actin was used as the loading control