JIP4/SPAG9 (D72F4) XP ® Rabbit mAb 5519T from Cell Signaling Technology

Product Specs
ItemJIP4/SPAG9 (D72F4) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $168.00 Inquire
Catalog Number5519T
ApplicationsWB, FCM, ICC, IF, IHC-p, IP
ReactivityHu, Ms, Rt, Mk
NCBI Gene AliasesCT89, HLC-6, HLC4, HLC6, JIP-4, JIP4, JLP, PHET, PIG6
NCBI Full Gene Namesperm associated antigen 9
TargetJIP4
Quantity20 µl
HostRabbit
ClonalityMonoclonal

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) JIP4 and RILPL1 utilize opposing motor force to dynamically regulate lysosomal tubulationJournal of Cell BiologyNovember 3, 2025Luis Bonet-Ponce, Tsion Tegicho, Nuria Fernandez-Martinez, Irene A. Rozenberg, Mia Ashriem, Alexandra Beilina, Jillian H. Kluss, Yan Li, Mark R. Cooksonused: mouse anti-FLAG M2 (F3165; Sigma-Aldrich; 1:500 for ICC and 1:10,000 for WB), rabbit anti-JIP4 (5519, 1:1,000; Cell Signaling Technology for WB and 1:100 for ICC), rabbit anti-LAMP1 (D2D11; 1:2,000; Cell

(2) TBCK-deficiency leads to compartment-specific mRNA and lysosomal trafficking defects in patient-derived neuronsbioRxivMarch 7, 2025Marco Flores-Mendez, Jesus A. Tintos-Hernández, Leonardo Ramos-Rodriguez, Leann Miles, Tsz Y Lo, Yuanquan Song, Xilma R. Ortiz-GonzálezHPA036791), rabbit anti-C12ORF4 (1:1000, Sigma, HPA037871), rabbit anti-JIP4 (1:1000, Cell signaling, 5519), rabbit anti-TRIM27 (1:1000, IBL, 18791), rabbit anti-CRYZL1 (1:500, Novus, NBP1–89367), OXPHOS cocktail

(3) Opposing actions of JIP4 and RILPL1 provide antagonistic motor force to dynamically regulate membrane reformation during lysosomal tubulation/sorting driven by LRRK2bioRxivAugust 28, 2024Luis Bonet-Ponce, Tsion Tegicho, Alexandra Beilina, Jillian H. Kluss, Yan Li, Mark R. Cooksonfor immunocytochemistry (ICC) and 1:10,000 for western-blot (WB)), rabbit anti-JIP4 (Cell Signaling Technology; 5519, 1:1000 for WB and 1:100 for ICC), rabbit anti-LAMP1 (Cell Signaling Technologies, D2D11; 1:2000 for WB

(4) A Non-Active-Site Inhibitor with Selectivity for Zika Virus NS2B-NS3 ProteaseACS Infectious DiseasesFebruary 9, 2024Kristalle G. Cruz, Maureen Hill Hill Eron, Sparsh Makhaik, Sergey Savinov, Jeanne A. Hardyfrom lot 2, catalog #8089, eIF4G1 was from lot 4, catalog #2498, SPAG9/JIP4 was from lot 1, catalog #5519, and CycA was from lot 2, catalog #2175. The membrane was washed and then incubated with secondary antibody

(5) VEGF and EGFR signaling pathways are involved in the baicalein attenuation of OVA-induced airway inflammation and airway remodeling in miceRespiratory ResearchJanuary 4, 2024Wang Peng, Qinxuan Xia, Yue Zhang, Danfeng Cao, Xiangrong Zheng(1:1000, #4267S, Cell Signaling Technology), and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH,1:1000, #5519, Cell Signaling Technology) at 4 °C. After washing with TBS-T buffer, the membranes were incubated with

(6) Axonal transport of autophagosomes is regulated by dynein activators JIP3/JIP4 and ARF/RAB GTPasesJournal of Cell BiologyDecember 4, 2023Sydney E. Cason, Erika L.F. Holzbaurintermediate chain antibody (Mouse MAB1618) plus JIP3 antibody (Rabbit ab196761), JIP4 antibody (Rabbit, 5519; Cell Signalling), or no second 1° antibody (negative control). Both 2° antibodies (Mouse and Rabbit)

(7) Directing LRRK2 to membranes of the endolysosomal pathway triggers RAB phosphorylation and JIP4 recruitmentNeurobiology of diseaseAugust 1, 2022Kluss JH, Bonet-Ponce L, Lewis PA, Cookson MR.anti-cyclophilin B (Abcam, cat #ab16045, 1:2,000 for WB), rabbit anti-JIP4 (Cell Signaling Technology, cat#5519, 1:100 for ICC), rabbit anti-LC3B (Cell Signaling Technology, cat#3868, 1:2,000 for WB), mouse anti-p62

(8) JIP4 is recruited by the phosphoinositide-binding protein Phafin2 to promote recycling tubules on macropinosomesJournal of Cell ScienceJuly 15, 2021Kia Wee Tan, Viola Nähse, Coen Campsteijn, Andreas Brech, Kay Oliver Schink, Harald StenmarkS3C–E. Antibodies and staining reagents Antibodies and reagents used were anti-JIP4 (Cell Signaling, cat #5519, western blotting 1:1000, immunofluorescence 1:100 or 1:500; Fig. 2C). Anti-Phafin2 (Sigma-Aldrich HPA024829;

(9) LRRK2 mediates tubulation and vesicle sorting from lysosomesScience advancesNovember 1, 2020Bonet-Ponce L, Beilina A, Williamson CD, Lindberg E, Kluss JH, Saez-Atienzar S, Landeck N, Kumaran R, Mamais A, Bleck CKE, et al.for immunocytochemistry (ICC) and 1:10,000 for western-blot (WB)], rabbit anti-JIP4 (Cell Signaling Technology; 5519, 1:1000 for WB and 1:100 for ICC), rat anti-LAMP1 [Developmental Studies Hybridoma Bank (DSHB), 1D4B;

(10) LncRNA H19 promotes the committed differentiation of stem cells from apical papilla via miR-141/SPAG9 pathwayCell Death & DiseaseFebruary 1, 2019Zehan Li, Ming Yan, Yan Yu, Yanqiu Wang, Gang Lei, Yin Pan, Na Li, Romila Gobin, Jinhua YuCell Signaling Technology), JNK (#9252, Cell Signaling Technology), p-JNK (#9255, Cell Signaling Technology), SPAG9 (#5519, Cell Signaling Technology) and GAPDH (#2118, Cell Signaling Technology)]. Membranes were washed with

(11) SPAG9 controls the cell motility, invasion and angiogenesis of human osteosarcoma cellsExperimental and therapeutic medicineFebruary 1, 2016Yang X, Zhou W, Liu S.and incubated overnight at 4˚C with the following antibodies: Rabbit anti‑SPAG9 (1:1,000; cat. no. 5519), rabbit anti‑MMP‑2 (1:1,000; cat. no. 4022), rabbit anti‑MMP‑9 (1:1,000; cat. no. 3852) (all Cell Signaling Technology

(12) Corticospinal tract-sparing intensity-modulated radiotherapy treatment planningReports of Practical Oncology & RadiotherapySeptember 1, 2014Hiroshi Igaki, Akira Sakumi, Akitake Mukasa, Kuniaki Saito, Akira Kunimatsu, Yoshitaka Masutani, Shunya Hanakita, Kenji Ino, Akihiro Haga, Keiichi Nakagawa, et al.(cGy) 6951 6802 6438 6768 6711 6518 6782 6697 6460 6798 6699 6500 PTV2 D98% (cGy) Dmean (cGy) D2% (cGy) 6573 5564 4648 6481 5480 4915 6440 5519 4802 6427 5485 4960 CST-R D98% (cGy) Dmean (cGy) V50 Gy (%) V40 Gy (%) V30 Gy (%) 4461 3860 0 28.89 96.87 5593 5039 71.99 96.57 97.74 – – – – – – – – – – CST-L D98%

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JIP4/SPAG9 (D72F4) XP ® Rabbit mAb from Cell Signaling Technology

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JIP4/SPAG9 (D72F4) XP ® Rabbit mAb from Cell Signaling Technology

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