Fig 2: SETD2 and H3K36me3 peak in early growing oocytes before declining during late oocyte growth. (A, C) Representative IF images showing SETD2 (A) and H3K36me3 (C) (red, grey single channel) in primordial, primary, secondary, early antral and antral follicle oocytes from wildtype adult mouse ovaries. The oocyte nucleus is defined by Lamin B1 (green, dashed lines), and DNA is shown by DAPI (blue). White boxes indicate regions shown at higher power in images on the right. Yellow arrowheads denote primordial follicle oocyte nuclei. Images are representative of multiple planes in both ovaries from three biological replicates. Scale bars represent 50 μm (left), and 10 μm (middle, right). (B, D) Quantification of SETD2 (B) and H3K36me3 (D) within oocyte nuclei of primordial, primary, secondary, early antral and antral follicles from wildtype adult mouse ovaries. Values represent average nuclear staining intensity with average cytoplasmic fluorescence removed to correct for non-specific background staining. Error bars represent mean ± SD. (B) ns: not significant, *P < 0.05, **P < 0.01, ****P < 0.0001, one-way ANOVA plus Tukey’s multiple comparisons test. N = 94 primordial follicle oocytes, N = 56 primary follicle oocytes, N = 32 secondary follicle oocytes, N = 17 early antral follicle oocytes, and N = 13 antral follicle oocytes, in total from three biological replicates. (D) ns: not significant, *P < 0.05, ***P < 0.001, Kruskal-Wallis test with Dunn’s multiple comparisons test. N = 82 primordial follicle oocytes, N = 47 primary follicle oocytes, N = 21 secondary follicle oocytes, N = 11 early antral follicle oocytes, and N = 6 antral follicle oocytes, in total from three biological replicates