Fig 2: Sirt3 depletion increases SOD2 acetylation and partially prevents antidepressant-, anxiolytic-like effects as well as antioxidant effects. (A). Immobility time in FST. (B,C) Rearing frequency (B) and time spent in the central area (C) in OFT. (D) Percentage of time spent in the open arms in EPM. (E–G) the membrane potential and antioxidant activity of mitochondria isolated from mouse hippocampal tissue of all experiment groups. Statistic results of mitochondrial membrane potential (E) were determined by the ratio between the fluorescence intensity at 590 nm (red fluorescence of polymer) and 530 nm (green fluorescence of monomer) after JC-1 staining. The antioxidant activity of mitochondria was reflected by the statistic result of SOD activity (F) and normalized statistic results of total antioxidant capability (G). (H) Representative immunoblot of protein levels of Ac-K68-SOD2 and SOD2 in the hippocampal mitochondria from mice in six experiment groups. (I) SOD2-K68 acetylation was normalized by SOD2. n = 12 per group in (A–G), n = 4 per group in (I) Data are represented as mean ± SEM. Two-way ANOVA analysis with Sidak’s multiple comparisons test or Mann–Whitney test: * p < 0.05, ** p < 0.01.

Fig 3: The gene and protein expression of SIRT3 in NSCLC and adjacent tissue. (a) The mRNA expression of SIRT3 was higher in NSCLC tissue than in adjacent tissue. (b) The protein expression of SIRT3 was higher in NSCLC tissue than in adjacent tissue n = 6, ∗∗p < 0.01.

Fig 4: Involvement of Sirt3 in perampanel‐induced protection. (A) The perampanel‐induced decrease in LDH release after TNI or glutamate treatment was prevented by Si‐Sirt3 transfection. (B) The perampanel‐induced decrease in apoptotic rate after TNI or glutamate treatment was prevented by Si‐Sirt3 transfection. Error bars indicate SEM (n = 6). # p < 0.05

Fig 5: Interrelations among PINK1, Parkin, BNIP3, BNIP3L/NIX, DRP1, MFN2, SIRT3, and GPX4.Thick solid arrows show the action of mitochondrial dynamics, thin solid lines demonstrate the impact on ROS production, and dotted lines indicate indirect influence of the ROS level. Made in Biorender.