Fig 2: The NF-κB inhibitor PDTC suppressed the function of oeTRIM27 in Caki-1 cells. A & B. oeTRIM27 significantly upregulated the relative mRNA and protein levels of TRIM27 in Caki-1 cells. ***p < 0.001 vs oeNC. The mRNA levels of TRIM27 was used 2−ΔΔCt method and normalized to that of GAPDH. C. The proliferation of oeTRIM27 transfected cells was deeply downregulated in the presence of the inhibitor PDTC. *p < 0.05 vs oeNC + DMSO, ***p < 0.001 vs oeNC + DMSO;!p < 0.05 vs oeTRIM27 + DMSO,!!p < 0.01 vs oeTRIM27 + DMSO,!!!p < 0.001 vs oeTRIM27 + DMSO. D. The inhibitor PDTC significantly reduced the apoptosis of oeTRIM27 transfected cells. *p < 0.05 vs oeNC + DMSO, ***p < 0.001 vs oeNC + DMSO;!!!p < 0.001 vs oeTRIM27 + DMSO. E. The nuclear translocation of NF-κB and IkBa in oeNC or oeTRIM27 transfected cells were deeply suppressed by the inhibitor PDTC. ***p < 0.001 vs oeNC + DMSO;!!!p < 0.001 vs oeTRIM27 + DMSO. F. Correlation analysis between TRIM27 and NF-κB in human renal cancer tissues (n = 20)

Fig 3: TRIM27 is upregulated and correlated with poor prognosis in human renal cancer. A. The mRNA level of TRIM27 was upregulated in renal cancer. Data were collected from the TCGA database. ***p < 0.001 vs pare-non-cancer tissues B. Prognosis of RCC (KIRC) patients with high or low expression of TRIM27 derived from the TCGA database. C. The relative mRNA level of TRIM27 was upregulated in human renal cancer tissues. n = 20, ***p < 0.001 vs pare-non-cancer tissues. D–F. KEGG, REACTOME, and DUTTA analysis indicated that cell apoptosis was positively correlated with elevated TRIM27 expression in RCC from the database GSE53757. NES: Normalized enrichment score

Fig 4: TRIM27 silencing reduced the tumorigenicity of human Caki-2 cells in vivo. A & B. Knockdown of TRIM27 in Caki-2 cells deeply suppressed the tumor volume and weight in vivo. **p < 0.01 vs siNC, ***p < 0.001 vs siTRIM27. C. TUNEL staining assays were used to determine tumor cell apoptosis. D. TUNEL quantification of siNC and siTRIM27 tumors, ***p < 0.001 vs siNC

Fig 5: TRIM27 silencing suppressed the proliferation and promoted apoptosis of human renal cells. A & B. TRIM27 siRNAs deeply suppressed the relative mRNA and protein content of TRIM27 in human Caki-2 and 786–0 cells, respectively. ***p < 0.001 vs siNC. The mRNA levels of TRIM27 was used 2−ΔΔCt method and normalized to that of GAPDH. C & D. siTRIM27–1 and siTRIM27–2 significantly suppressed the proliferation of Caki-2 and 786–0 cells. *p < 0.05 vs siNC, ***p < 0.001 vs siNC. E. Apoptosis of Caki-2 and 786–0 cells was significantly increased following transfecting with siTRIM27–1 and siTRIM27–2 at 48 h, respectively. ***p < 0.001 vs siNC. F & G. Western blot was used to examine the protein contents of TRIM27, cleaved caspase-3, NF-κB (cytoplasm), NF-κB (nuclear) and IkBa in Caki-2 cells (F) and 786–0 cells (G) transfected with siNC, siTRIM27–1, or siTRIM27–2 at 48 h respectively. *p < 0.05 vs siNC, **p < 0.01 vs siNC, ***p < 0.001 vs siNC