Fig 1: Alox15 was identified as a key target of Cyn. (A) The top 10 Cyn targets were visualized. (B) 3D-molecular docking of Cyn with OTUD7B. (C) Interaction of the Cyn with Alox15 by biolayer interferometry. (D) The protein expressions of Alox15 in cortex of cerebral ischemia reperfusion model with Cyn treatment. (E) Statistical analysis of the protein expression intensity of Alox15. (F) Immunofluorescence for Alox15 and counterstaining with DAPI in the cortex regions in the ipsilateral side of tMCAO. ** represents p < 0.01. n = 3. The control group was used for comparison. Data are shown as mean ± SD.