Fig 2: Supernatant liquid of transfected endometrial cancer cell lines affects the proliferative capacity of HUVEC. (a) MTT experiment: effect of the proliferation of HUVEC in culture with endometrial cancer supernatant transfected by ATAD2. (b) The cloning experiment reflects the proliferation between different groups. (c) Quantitative plots of clone formation in different groups.

Fig 3: Immunohistochemical staining of targeted proteins.(A) T1 colorectal cancer (CRC) samples from a set of 47 cases were used to verify the abundance of ABI1, ITPR2, RHOT2, ATAD2, and ISLR. The scores that represent the sum of the intensities and percentage of protein staining in the lymph node metastasis (LNM)-positive or LNM-negative patients are shown as a heat map. (Histological images were obtained using a × 40 objective, scale bars, 100um). The receiver operating characteristic (ROC) curve of each protein was built by their immunohistochemistry (IHC) score. (B) ROC curve of the five proteins classifier using IHC score by binary logistic regression. (C) The overall survival of patients with colon cancer was analyzed on the basis of The Cancer Genome Atlas (TCGA) database. (D) Correlations between RHOT2 activities and protein abundances in the T1 CRC cohort. (E) Gene set enrichment analysis (GSEA) pathways using the single-gene method of RHOT2. (F) The RHOT2 protein expression in human colon cancer cells (SW480, HT29, HCT-116, RKO, DLD1, and LoVo) was measured by western blotting. (G) The protein expression of RHOT2 in HT29. (H & I) The migration ability of HT29 cells was detected by transwell assay (images were obtained using a × 20 objective, scale bars, 100um) (Student’s t-test). Figure 4—source data 1.Immunohistochemistry (IHC) staining score. Figure 4—source data 2.RHOT2 western blot source images.

Fig 4: Upregulation of ATAD2 promotes tumor growth and angiogenesis. (a) Transplant tumor growth in mice after injection of ATAD2 overexpression, knockdown, and control of endometrial cancer cells. (b–d) Expression of ATAD2, VEGF, and MVD in knockdown ATAD2 tumors. (e) Mouse transplant tumor growth curve. (f) Immunohistochemical images corresponding to knockdown of ATAD2 (magnification, 200x). (g) The picture of immunohistochemistry of transplanted tumor when ATAD2 was overexpressed above (magnification, 200x). (h–j) Overexpression of ATAD2 increases the expression of VEGF and MVD in transplanted tumor models.

Fig 5: ATAD2 is associated with TIL infiltration in UCEC. (a) GO enrichment analysis of the biological processes of ATAD2 coexpressed genes. (b) KEGG enrichment analysis of the signaling pathways of ATAD2 coexpressed genes. (c) TIMER online website assessing ATAD2 correlation with immune infiltration in UCEC. (d) Three lymphocytes most significantly correlated with ATAD2 immune infiltration analysis in UCEC based on TISIDB. (e) GEPIA analysis of ATAD2 correlation with classical immune checkpoint inhibitors.