NDUFB8 (E7U3O) Rabbit mAb 73951T from Cell Signaling Technology

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Advanced science (Weinheim, Baden-Wurttemberg, Germany)
May 01 2026

Fig 1: TRAP5 regulated macrophage metabolic functions and polarization in response to OPN. (A) Cell viability of BMDMs after 24 h exposure to OPN (Osteopontin) with or without TRAP5i in a dose dependent manner (n = 6 per group). (B) Acp5 mRNA levels in BMDMs stimulated with OPN ± TRAP5i; (C) NAD+/NADH ratios in BMDMs after OPN stimulation with or without TRAP5i (n = 3 independent experiments per group). (D) Measurement of ATP in BMDMs treated with OPN ± TRAP5i (n = 3 independent experiments per group). (E) Representative immunoblots of mitochondrial respiratory chain/ATP synthase subunits (NDUFB8, UQCRC2, MT‐CO1, ATP5A1, SDHB) in BMDMs treated with OPN ± TRAP5i, GAPDH shown as a loading control. (F) Densitometry analysis was performed to quantify protein expression, normalized to GAPDH (n = 3 independent experiments per group). (G) Relative mRNA Transcript of glutaminolysis enzymes (Gls, Glud1) (n = 3 independent experiments per group). (H) Relative mRNA Transcript of Cd68, Ccl2, Cd86 (n = 3 independent experiments per group). (I) Relative mRNA Transcript of Ym1, Il10, Tgfb1, Retnla, Mrc1, Mr upon OPN stimulation, following TRAP5 inhibitor treatment (n = 3 independent experiments per group). Data are shown as mean ± SEM. Group comparisons in (A) were assessed by one‐way ANOVA, and those in (B–D, F–I) were assessed by two‐way ANOVA. (*p < 0.05, ** p < 0.01, ***p < 0.001, ****p < 0.0001).
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bioRxiv
July 01 2026

Fig 2: pS754 Stat3 limits Stat3 mitochondrial targeting and increases macrophage cell deathA) Immunoblot untreated iBMDMs stably expressing 3X-Flag GFP, 3X-Flag STAT3, or 3X-Flag MTS-STAT3. Actin was used as a loading control.B) Biochemical fractionation and immunoblot of tetracycline-inducible overexpression iBMDMs expressing 3X-Flag GFP, 3X-Flag STAT3, or 3X-Flag MTS-STAT3 for 8hr. Anti-Flag antibody was used to measure mitochondria association before and after treatment with 50μg/ml proteinase K (PK) to remove cytosolic contaminants from the mitochondria. Ndufb8 is an inner mitochondrial protein protected from PK. Outer membrane protein Tom20 is degraded upon PK treatment.C) Cell death in iBMDMs stably expressing 3X-Flag GFP, 3X-Flag STAT3, or 3X-Flag MTS-STAT3 treated with 10 μM ABT-737.D) Immunoblot analysis of untreated RAW 264.7 macrophages stably expressing 3X-Flag GFP, 3X-Flag STAT3, 3X-Flag STAT3 S754A, or 3X-Flag STAT3 S754E. Actin was used as a loading control.E) As in (C) but with RAW 264.7 macrophages expressing 3X-Flag GFP, 3X-Flag STAT3, 3X-Flag STAT3 S754A, or 3X-Flag STAT3 S754E.F) Cell death in tetracycline-inducible iBMDMs expressing 3X-Flag-STAT3 S754E and the corresponding MTS-tagged construct following doxycycline induction (1 μg/mL, 8h) and 5μM ABT-737. Actin was used as a loading control.G) Biochemical fractionation and immunoblot analysis of untreated iBMDMs stably expressing 3X-Flag GFP, 3X-Flag STAT3, 3X-Flag STAT3 S754A, or 3X-Flag STAT3 S754E. Stat3 was probed to measure mitochondria association before and after treatment with 50μg/ml proteinase K (PK) to remove cytosolic contaminants from the mitochondria. Ndufb8 and Tom20 serve as controls as in (B).Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Statistical differences were determined for (C, E, F) using two-way ANOVA with Tukey’s post-test.

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NDUFB8 (E7U3O) Rabbit mAb

Cell Signaling Technology's NDUFB8 (E7U3O) Rabbit mAb is a Rabbit monoclonal antibody. The NDUFB8 (E7U3O) Rabbit mAb Antibody was generated using NDUFB8 as the antigen. It reacts with Homo Sapiens, Human, Mouse, and Rat. This antibody has been shown to work in applications such as: Western Blot.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

bioRxiv

NDUFB8 (E7U3O) Rabbit mAb from Cell Signaling Technology

Cell Signaling Technology

Citations (2)

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NDUFB8 (E7U3O) Rabbit mAb from Cell Signaling Technology

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