Fig 1: Analysis of the LMO4 protein amount depending on nimodipine and cisplatin treatment. A representative Western blot of three independent biological replicates of undifferentiated UB/OC−1 (a) and UB/OC−2 (b) cells as well as of differentiated UB/OC−1 (c) and UB/OC−2 (d) cells is shown. Fifty µg protein per sample was loaded and separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and blotted onto 0.2 µm nitrocellulose membranes as described in materials and methods section. Detection was performed by using specific antibodies. While the amount of the protein LMO4 was strongly reduced by cisplatin, there was an increase by pre-treatment with 10 µM and 20 µM nimodipine during 20 µM cisplatin.