Fig 2: The levels of colonic TLR4 and related protein expression in Tlr4fl/fl and Tlr4ΔIEC offspring mice with or without prenatal LPS exposure. (A) The levels of colonic TLR4 protein expression in offspring mice (n = 4 per group). (B) The levels of colonic CHUK, RELA, P-RELA, and PTGS2 protein expression in the four groups (n = 4 per group). (C) The TLR4, CHUK, and PTGS2 protein expression levels in the four groups were normalized to ACTB, and the P-RELA to RELA ratio was used to evaluate the proportion of P-RELA quantitatively (n = 4 per group; two-way ANOVA). Data were presented as mean ± standard error of the mean. The term “Interaction” denotes the effect of LPS in the Tlr4ΔIEC mice compared with Tlr4fl/fl mice. ns, not significant; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001. TLR4, toll-like receptor 4; LPS, lipopolysaccharide; CHUK, component of inhibitor of nuclear factor Kappa B kinase complex; PTGS2, prostaglandin-endoperoxide synthase 2; ACTB, actin beta.