Fig 1: MST2 regulates nucleolar transcription in response to γIR via H2BS14 phosphorylation Relative pre‐rRNA expression in HeLa cells at the indicated times after exposure to γIR. Expression of pre‐rRNA was normalised against GAPDH. Error bars represent the SD and derive from two independent experiments.HeLa cells were treated with the indicated siRNAs and exposed to γIR. 20 min after exposure pre‐rRNA expression was normalised against GAPDH. Error bars represent the SD and derive from two independent experiments.HeLa cells were treated with the indicated siRNAs, exposed to γIR and treated with 0.5 mM 5‐EU for 20 min. 5‐EU incorporation was quantified, and representative images from each condition are shown here and in Fig EV2F. Quantification of data derived from three independent experiments is shown on the right. Middle line represents the median and the boxes 25th and 75th percentiles. The whiskers mark the smallest and largest values.HeLa cells were transfected with H2B‐GFP or H2BS14D‐GFP. pre‐rRNA abudance was assessed with qPCR. Expression of pre‐rRNA was normalised against GAPDH. Error bars derive from two independent experiments and represent the SD.HeLa cells were transfected with H2B‐GFP or H2BS14A‐GFP and exposed to γIR. pre‐rRNA abudance was assessed with qPCR. Expression of pre‐rRNA was normalised against GAPDH. Error bars derive from two independent experiments and represent the SD.HeLa cells were exposed to γIR, fixed at the indicated times and stained for RCC1 and nucleolin. Representative images (left) and quantification (right) of cells with nucleolar staining of RCC1 are shown. Error bars represent the SD and derive from three independent experiments.Data information: DNA was stained with DAPI. Scale bars at 10 μm. Two‐tailed Student's t‐test (A, B, D–F) or Mann–Whitney test (C) was used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001.