Fig 1: IL-1β mediates macrophage-SMC communication. A Predicted ligand activities mediating BMDM-SMC communication using the NicheNet R package. IFNγ/LPS stimulated BMDM bulk RNA-seq datasets were downloaded from GEO (GSE103958). B Expression and cellular distribution of Il1a, Il1b, Il1r1 and Il1rap in scRNA-seq data. C Representative Western blot and quantification of the time course of p-YAP(S127), p-YAP(S397), total-YAP, SM22, and MYH11 protein levels in SMCs challenged with 20 ng/mL recombinant mouse IL-1β. D RT-qPCR of Ccl2 and Myh11 in SMCs treated with recombinant mouse IL-1β and/or PY-60. Data in are presented as mean ± SD. Two-way ANOVA is performed in (C&D). *p < 0.05, **p < 0.01, ***p < 0.001