S100A9 (D3U8M) Rabbit mAb 73425T from Cell Signaling Technology

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Military Medical Research
June 19 2023

Fig 1: Paquinimod ameliorates sepsis-induced immunosuppression by targeting S100ahigh monocytes. a-c Mice undergoing CLP surgery were given Paquinimod [(10 mg/(kg·d)] by oral gavage for 3 d or not, followed by isolation of circulating monocytes in sham or CLP mice at 72 h post-operation. Counter plots with quantitative bar charts showed the proportion of MHC-II−S100A9+ monocytes in various groups (a). Representative confocal immunofluorescence images of S100A9+ monocytes in each group (Scale bar = 50 μm, 20 μm) (b). Western blotting analysis indicated the protein expression of S100A9 (c). d The survival rates of mice from disparate groups were recorded and compared within 7 d post-CLP surgery, shown by Kaplan–Meier curve. e Representative images of HE staining exhibited the pathological alterations in multiple organs of mice, including lung, liver, kidney, and heart (Scale bar = 150 μm). f-i PBMCs and plasma were collected from CLP mice treated with or without Paquinimod. Histogram and quantitative bar charts indicated the percentage of CD3+ cells in different groups (f). Counter plots with quantitative bar charts were compared the proportion of CD3+CD4+ cells between groups (g). Representative counter plots with quantitative bar plots showed CD3+CD4+Foxp3+ Tregs proportion (h). Quantitative bar plots showed and compared circulating levels of IL-2, IL-4, IL-10, IFN-γ, TGF-β and the ratio of IFN-γ to IL-4 across each group (i). Statistics were by One-way ANOVA with Tukey HSD test for comparison of two groups (a, f–i). Statistics were calculated using survival curve comparison with log-rank test (d). Data are shown as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, One-way ANOVA, was performed in (a, f–i). CLP cecal ligation and puncture, MHC major histocompatibility complex, HE hematoxylin–eosin, PBMCs peripheral blood mononuclear cells, IL interleukins, IFN-γ interferon-γ, ANOVA analysis of variance, SD standard deviation, PAQ Paquinimod
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Nature communications
March 18 2022

Fig 2: Positive regulation loop between S100a9 and Cxcl12 amplifies oncogenic signals in Brca1-MT epithelial cells.a The Venn diagram analysis of upregulated genes in G600, Over-Expression-S100a9 in B477 cells (B477-OE-S100a9), and down-regulated genes in G600 cells expressing sgS100A9 (G600-sgS100a9) (n = 3 biological replicates/group). b KEGG Pathway analysis with 453 common (p value was calculated by hypergeometric test with KOBAS 3.0 website). c Top 20 differentially expressed genes by comparison of the gene expression profiles of four different group cells, including B477-Ctr (B1-3), B477-OES100a9 (P1-3), G600-Ctr (G1-3), and G600-sgS100a9 (G10-1-3) (the heatmap was drawn by Morpheus website and clustered by One minus Pearson Correlation). d, e Expressions of S100a9 and Cxcl12 at mRNA level by qPCR (d) at protein levels by western blotting (e) from four group cells in c. f Protein levels of S100a9, Cxcl12 and pStat3 in OE-S100a9-EMT6 cells, sgS100a9/OE-S100a9-EMT6, and sgCxcl12/OE-S100a9-EMT6 cells. g Protein levels of S100a9, Cxcl12 and pStat3 in OE-S100a9-EMT6 and G600 cells without or with S100A9 inhibitor, Tasquinimod (Tas-50 μM) by western blotting. h Protein levels of S100a9, Cxcl12, and pStat3 in WT (B477) cells with the expression of OE-S100a9, OE-S100a9/sgCxcl12, or OE-Cxcl12 by western blotting. i, k Protein levels of Cxcl12 in B477 (i) cells with OE-S100a9 and in G600 (k) cells expressing sgS100a9 at different time courses (0–48 h) and different amounts (0–4 μg) by western blotting. j, l Protein levels of S100a9 in B477 (j) cells with OE-Cxcl12 and in G600 (l) cells expressing sgCxcl12 at different time courses (0–48 h) and different amounts (0–4 μg) by western blotting. m Protein levels of S100a9, Cxcl12, and pStat3 in G600 cells after treating with Tas and FPS-ZM1, inhibitor for RAGE receptors on cell membranes by western blotting. n Protein levels of S100a9, Cxcl12, and pStat3 in B477 cells with the expression of shBrca1 and MT G600 cells with over-expression of Brca1 (OE-Brca1). o A diagram summarizing the relationship of Brca1, S100a9, Cxcl12 and pStat3 in Brca1-MT mammary epithelial cells, and their potential interaction with the surrounding immune microenvironment, which remains elusive. The data are expressed as means ± SD (d) and P values determined by one-way ANOVA followed by Tukey’s multiple comparisons. The experiments were independently repeated three times with similar results (d–n). Source data are provided as a Source data file.
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International journal of molecular sciences
January 20 2023

Fig 3: Blocking AMPK abrogates the S100A9-KO-mediated improvement of CLP-induced liver mitochondria dysfunction and reduction of glucose and lipid metabolism. (A) Representative images of O2 concentration change (blue line) and O2 flux per mass (red line) in groups. (B) Summarized data for the oxygen consumption capacity measured by high-resolution respirometry in CI leak, CI P, CI + CII P, CI + CII ETS, CII ETS (n = 6). (C) ATP production and (D) fluorescence (represent mitochondrial membrane potential) (n = 6). (E) Serum and (F) tissue levels of TG and FFA (n = 6). (G) Immunoblotting analysis of S100A9, p-AMPK, AMPK, ACC, GLUT4 (left) and quantification of the relative protein levels (right, n = 4). Data are shown as mean ± SEM, and n represents the number of mice in each group.
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Renal failure
December 01 2022

Fig 4: S100A8 and S100A9 are upregulated in the myocardial tissues of UCM rats. (A) The levels of BUN in rats of each group; (B) Levels of blood creatinine in rats of each group; (C) SBP in rats of each group; (D) The content of CK-MB in rats of each group; (E–G) The heart weight (E), body weight (F), and the ratio of heart weight to body weight (G) in rats of each group; (H) Representative images of HE staining and quantitation of cardiomyocyte area in rats of each group (scale bar: 50 µm); (I) Masson staining observation of myocardial fibrosis in each group (scale bar: 50 µm); (J) qRT-PCR and western blot detection of mRNA and protein expression of S100A8 and S100A9 in myocardial tissues of rats in each group. *p < 0.05 compared with the sham group. N = 6 rats/group. UCM: uremic cardiomyopathy; BUN: blood urea nitrogen; SBP: systolic blood pressure; CK-MB: creatine phosphokinase-MB; qRT-PCR: quantitative real-time polymerase chain reaction.
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International journal of molecular sciences
January 20 2023

Fig 5: Administration of S100A9 inhibitor ameliorates liver mitochondrial dysfunction through increasing AMPK-mediated glucose and lipid metabolism. (A) Representative images of O2 concentration change (blue line) and O2 flux per mass (red line) in groups. (B) Summarized data for the oxygen consumption capacity measured by high-resolution respirometry in CI leak, CI P, CI + CII P, CI + CII ETS, CII ETS (n = 6). (C) ATP production and (D) fluorescence (represent mitochondrial membrane potential) (n = 6). (E) Immunoblotting analysis of p-AKT, AKT, p-AMPK, AMPK, ACC, GLUT4 (left) and quantification of the relative protein levels (right, n = 4). Data are shown as mean ± SEM, and n represents the number of mice in each group.

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Cell Signaling Technology for
S100A9 (D3U8M) Rabbit mAb

Cell Signaling Technology's S100A9 (D3U8M) Rabbit mAb is a Rabbit monoclonal antibody. The S100A9 (D3U8M) Rabbit mAb Antibody was generated using S100A9 as the antigen. It reacts with Mouse, and Rat. This antibody has been shown to work in applications such as: Precipitation, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot.

Product Specs
ItemS100A9 (D3U8M) Rabbit mAb
CompanyCell Signaling Technology
Price $162.00 Inquire
Catalog Number73425T
ClonalityMonoclonal
ReactivityM, R
ApplicationsW, IP, IHC-Bond, IHC-P, IF-F, FC-FP
HostRabbit
Quantity20 µl
TargetS100A9 mouse
NCBI Full Gene NameS100 calcium binding protein A9 (calgranulin B)
NCBI Gene Aliases60B8Ag, BEE22, Cagb, GAGB, L1Ag, MRP14, p14

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(1) Single-cell insights into trophoblast heterogeneity and adaptive dysfunction in selective fetal growth restrictionCommun BiolMarch 17, 2026Yan Bi, Jiawen Yang, Xiaoyu Li, Yuhong Lin, Yucheng Hu, Xiang Ying, Li Gao, Yanlin WangTP63 (CST, #39692, 1:500), SEMA6D (abcam, ab198745, 1:100), LDHA (CST, #3582S, 1:200), S100A9 (CST, #73425, 1:100), and CDH1 (CST, #14472S, 1:200). Sequential detection was performed using HRP-conjugated secondary

(2) Purified Diets Lacking Fermentable Fiber Reduce Microbial Diversity, Alter Epithelial Transcriptome, and Exacerbate ColitisNutrientsMarch 11, 2026Emma Griffith Thomas, Beulah Favour Ortutu, Jacob Connor Watson, Ethan Ong, Kaitlyn Eileen Blankley, Angela Meaurio Martin, Smriti Shankar, Dongmei Zhang, Devon Joseph Boland, Chia-Shan Wuwere first validated and then used at the following dilutions: 1:50 for anti-S100A9 rabbit antibody (73425S) and 1:200 for anti-GAPDH rabbit antibody (2118S). Both antibodies were purchased from Cell Signaling

(3) PINK1 dysfunction in hepatocellular carcinoma fosters immune evasion and disease progression by promoting neutrophil infiltrationJ Immunother CancerDecember 18, 2025Zhiyun Gu, Tongwang Yang, Yuanli Ni, Yuanze Zhou, Cheng Qian, Na Zhuang(Avantor). The following primary antibodies were used: PINK1 (sc-517353, Santa Cruz, 1:100) and S100A9 (#73425, CST, 1:800). In all assays, the primary antibody in the negative control slides was replaced with PBS. Single-cell

(4) Infiltrating macrophages replace Kupffer cells and play diverse roles in severe alcohol-associated hepatitisCellular & Molecular ImmunologySeptember 17, 2025Yang Wang, Yukun Guan, Dechun Feng, Luca Maccioni, Maria A. Parra, Brandon Peiffer, Bryan Mackowiak, Takashige Kuwabara, Kiyoshi Mori, Masashi Mukoyama, et al.No.,Dilution IBA1,Wako,019-19741,1:1000 MARCO,Invitrogen,PA5-64134,1:200 CD74,OriGene,AM32811PU-T,1:200 CD11c,CST,45581,1:200 LGMN,CST,93627S,1:200 SPP1,CST,88742,1:200 APOE,CST,13366,1:200 CD36,Invitrogen,MA1-46000,1:200 MPO,DSHB,CPTC-MPO-1,1:200 S100A9,CST,73425,1:200 F4/80,CST,70076,1:200 TIM4,CST,75484,1:200 CD68,Biocare Medical,KP1,1:200

(5) Myeloid-specific S100A8/A9 deficiency attenuates atrial fibrillation through prevention of TLR4/NF-kB-mediated immune cell recruitment and inflammationFrontiers in ImmunologySeptember 4, 2025Qing Wang, Hua Shen, Jing Wang, Guifang Wang, Yufeng ZhangH&E or Masson’s trichrome. IHC used anti-S100A8 (1:200, Abcam, ab92331), anti-S100A9 (1:200, CST, #73425), CD11b (1:200, BioLegend, #480109), and Ly6C (1:200, BioLegend) with DAB visualization. 2.12 Western blotting

(6) Neutrophil Mobilization Triggers Microglial Functional Change to Exacerbate Cerebral Ischemia‐Reperfusion InjuryAdvanced ScienceSeptember 1, 2025Huijuan Jin, Zhifang Li, Senwei Tan, Qinghui Xiao, Qingcan Li, Jiao Ye, Yifan Zhou, Yan Wan, Qiang Liu, Bijoy K Menon, et al.transferred PVDF membranes was incubated with primary antibodies overnight at 4 °C: MRP14 (1:1000, 73425, Cell Signaling Technology), Occludin (1:1000, 13409‐1‐AP, Proteintech), ZO‐1 (1:1000, 21773‐1‐AP, Proteintech),

(7) Single-cell atlas of human liver and blood immune cells across fatty liver disease stages reveals distinct signatures linked to liver dysfunction and fibrogenesisNature immunologySeptember 1, 2025Martin OP, Wallace MS, Oetheimer C, Patel HB, Butler MD, Wong LP, Huang P, Elbaz J, Costentin C, Salloum S, et al.in FFPE liver sections staining using primary rabbit mAb (D3U8M, Cell Signaling Technology, cat. no. 73425) 1:800, overnight at 4 °C. Measurement of soluble CD163 in patient serum To assess the macrophage

(8) SMAD4 Deficiency Promotes Pancreatic Cancer Progression and Confers Susceptibility to TGFβ InhibitionCancer ResearchAugust 15, 2025Gilbert Z. Murimwa, Natalie E. Williams, Dina Alzhanova, Amir Mohammadi, Jill M. Westcott, Francisca Beato, Ruifan Dai, Luis Nivelo, Francesca Rossi, Henry K. Fleming, et al.Cell Signaling Technology, RRID: AB_2799357), E-cadherin (3195, Cell Signaling Technology, RRID: AB_2291471), S100A9 (73425, Cell Signaling Technology, RRID: AB_2799839), LRRC15 (50546, Cell Signaling Technology, RRID: AB_3674603),

(9) Neutrophil–Endothelium Interaction Mediated by S100A9 Promotes Pulmonary Vascular Remodeling During Pulmonary HypertensionAdvanced ScienceJune 10, 2025Yu Guo, Zhengqiang Gao, Ruoyang Zhang, Huanyu Long, Muzhi Zhang, Lin Liu, Zhe An, Yuezhe Shi, Ye Cui, Yufeng Jia, et al.incubation with primary antibodies against neutrophil elastase (ab68672, 1:2500, Abcam) and S100A9 (#73425, 1:200, CST), followed by HRP‐conjugated secondary antibodies and DAB development. For IF staining,

(10) Fh15 Reduces Colonic Inflammation and Leukocyte Infiltration in a Dextran Sulfate Sodium-Induced Ulcerative Colitis Mouse ModelCellsMay 29, 2025María Del Mar Figueroa-Gispert, Claudia M. Ramos-Lugo, Carlimar Ocasio-Malavé, Rizaldy P. Scott, Jared T. Ahrendsen, Mercedes Gomez-Samblas, Antonio Osuna, Stephanie M. Dorta-Estremera, Ana M. EspinoNo.,Vendor CD3εF4/80,T-cellsMacrophages,RabbitRabbit,1:1001:100,ab1666970076S,AbcamCST LY6G,Neutrophils,Rabbit,1:75,87048S,CST S100A9,Calcium binding protein A9,Rabbit,1:800,73425S,CST Primary antibodies (IgGs) used for chromogenic immunohistochemistry (IHC).

(11) S100A9 as a potential novel target for experimental autoimmune cystitis and interstitial cystitis/bladder pain syndromeBiomark ResMay 9, 2025Jiang Zhao, Mi Zhou, Chengfei Yang, Yang-Wuyue Liu, Teng Yang, Bishao Sun, Benyi Li, Ji Zheng, Shuangshuang Dai, Zhenxing Yang, et al.antibodies used in this investigation were as follows: rabbit monoclonal anti-S100A9 antibody (1:400, 73425, CST, USA), mouse monoclonal anti-CD4 antibody (1:100, Thermo Fisher, USA), mouse monoclonal anti-68

(12) Macrophage-derived S100A9 promotes diabetic cardiomyopathy by disturbing mitochondrial quality control via STAT3 activationInternational journal of biological sciencesJanuary 1, 2025Huo S, Wang M, Du M, Ren B, Yang T, Peng L, Jiang Y, Peng D, Men L, Shi W, et al.1% goat serum for 1 hour at 37°C, staining was performed as above with anti-S100A9 (1:100 dilution, #73425, CST), anti-α-actinin (1:100 dilution, 66895-1-Ig, Proteintech), anti-CD11b (1:100 dilution, 66519-1-Ig,

(13) Brain Resident Ly6Chi Monocytes Are Necessary for Maintaining Adult Hippocampal Neurogenesisaging and diseaseDecember 3, 2024Yiran Huang, Nan Gao, Boren Liu, Weili Luo, Jianfei Chen, Yan Chen, Yong Bi, Zikai Zhouanti-mouse Iba1,proteintech,10904-1-AP Rat anti-mouse CD31,abcam,AB256569 Rabbit anti-mouse S100A9,CST,73425S Anti-rabbit IgG,Abbkine,A23310 Anti-rat IgG,Abbkine,A23240 FITC anti-mouse NK-1.1,Biolegend,108706 PE/Cyanine5 anti-mouse F4/80

(14) FLT1 activation in cancer cells promotes PARP-inhibitor resistance in breast cancerEMBO molecular medicineAugust 1, 2024Tai Y, Chow A, Han S, Coker C, Ma W, Gu Y, Estrada Navarro V, Kandpal M, Hibshoosh H, Kalinsky K, et al.Cell Signaling Technology), FOXP3 (1:100, #12653, Cell Signaling Technology), murine S100A9 (1:1000, #73425, Cell Signaling Technology), FLT4 (1:250, #AF743, R&D Systems), and phospho-Stat3 (S727) (1:100, #9134,

(15) Hair follicles modulate skin barrier functionCell reportsJuly 23, 2024Ford NC, Benedeck RE, Mattoon MT, Peterson JK, Mesler AL, Veniaminova NA, Gardon DJ, Tsai SY, Uchida Y, Wong SY.,R&D Systems,Cat # AF2624 Rabbit anti-Rab11a,Cell Signaling,Cat # 2413 Rabbit anti-S100a9,Cell Signaling,Cat # 73425 Rabbit anti-Sprr2d,Proteintech,Cat # 23046–1-AP InVivoMAb anti-mouse IL-17A,BioXCell,"Cat # BE0173,

(16) Mitochondrial transplantation confers protection against the effects of ischemic stroke by repressing microglial pyroptosis and promoting neurogenesisNeural regeneration researchJune 1, 2024Sun L, Zhao Z, Guo J, Qin Y, Yu Q, Shi X, Guo F, Zhang H, Sun X, Gao C, et al.Novus, Littleton, CO, USA, Cat# NBP2-15189), rabbit anti-S100A9 (1:1000, Cell Signaling Technology, Cat# 73425, RRID: AB_2799839), rabbit anti-NLRP3 (1:1000, Cell Signaling Technology, Cat# 15101, RRID: AB_2722591),

(17) The heterogeneity of signaling pathways and drug responses in intrahepatic cholangiocarcinoma with distinct genetic mutationsCell death & diseaseJanuary 11, 2024Feng Y, Zhao M, Wang L, Li L, Lei JH, Zhou J, Chen J, Wu Y, Miao K, Deng CX.Cell Signaling Technology, 70076), CXCL9 (1:500, Invitrogen, PA5-79115), S100A9 (1:300, Cell Signaling Technology, 73425), and PD-L1 (1:300, Cell Signaling Technology, 13684). Western blot analysis Total protein lysates

(18) KRAS allelic imbalance drives tumour initiation yet suppresses metastasis in colorectal cancer in vivoNature CommunicationsJanuary 2, 2024Arafath K. Najumudeen, Sigrid K. Fey, Laura M. Millett, Catriona A. Ford, Kathryn Gilroy, Nuray Gunduz, Rachel A. Ridgway, Eve Anderson, Douglas Strathdee, William Clark, et al.#14-9766-82), CD8 (1:500, eBisoscience #14-0808-82), F4/80 (1:200, Abcam #ab6640) and S100A9 (1:1500, CST #73425) was performed on formalin-fixed intestinal sections using standard protocols. Secondary antibodies

(19) Single-cell transcriptome profiling of sepsis identifies HLA-DR(low)S100A(high) monocytes with immunosuppressive functionMilitary Medical ResearchJune 19, 2023Yao RQ, Zhao PY, Li ZX, Liu YY, Zheng LY, Duan Y, Wang L, Yang RL, Kang HJ, Hao JW, et al.for 1 h. Afterwards, cells were stained with anti-S100A9 antibody (1:200; Cell Signaling Technology, 73425) followed by incubation with fluorochrome-conjugated secondary antibody after PBS wash for 3 times.

(20) Co-Stimulation of AGEs and LPS Induces Inflammatory Mediators through PLCγ1/JNK/NF-κB Pathway in MC3T3-E1 CellsCellsMay 13, 2023Tanabe N, Tomita K, Manaka S, Ichikawa R, Takayama T, Kawato T, Ono M, Masai Y, Utsu A, Suzuki N, et al.Cat#5690), phosphorylated PLCγ1 (p-PLCγ1; Cat#14008), S100 calcium-binding protein A9 (S100A9; Cat#73425) (Cell Signaling Technology, Danvers, MA, USA), or β-actin (Cat#sc-47778, Santa Cruz Biotechnology,

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Military Medical Research

Nature communications

International journal of molecular sciences

Renal failure

International journal of molecular sciences

S100A9 (D3U8M) Rabbit mAb from Cell Signaling Technology

Cell Signaling Technology

Citations (41)

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S100A9 (D3U8M) Rabbit mAb from Cell Signaling Technology

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