Fig 1: scRNAseq profiling of ecDNA and HSR-driven PDAC TME with IHC validation.(A) Schematic overview of the scRNAseq workflow.(B) UMAP visualization of all cells identified by scRNAseq from EC1 and HSR1 tumors.(C) Cell composition analysis of stromal and immune cell populations. Fisher’s exact test with FDR correction. NS indicates not significant.(D) UMAP visualization of CAF subclusters.(E) Cell composition analysis of CAF subclusters. Colors correspond to those in Figure 2D. Fisher’s exact test for the overall p-value, pairwise Fisher’s exact test with Benjamini-Hochberg correction for specific cell types.(F) Representative images of POSTN IHC staining showing both low- and high-magnification views. Scale bars 2.5 mm (low magnification), 50 μm (high magnification).(G) Quantification of POSTN IHC staining. Each color within a group represents an individual mouse. Four non-overlapping regions from the same section were analyzed and shown in the same color. Sample sizes: EC, n = 6; HSR, n = 6. Wilcoxon test.(H) Cell composition analysis of all Ptprc+ immune cells. Fisher’s exact test with FDR correction. NS indicates not significant.(I) Representative images of CD3E IHC staining showing low- and high-magnification views. Scale bars: 5 mm (low magnification) and 50 μm (high magnification). Red letter T and circles indicate the tumor area in the pancreas.(J) Quantification of CD3E IHC staining. Each color within a group represents an individual mouse. Four non-overlapping regions from the same section were analyzed and shown in the same color. Sample sizes: EC, n = 7; HSR, n = 8. Wilcoxon test.(K) Representative images of CD8A IHC staining showing low- and high-magnification views. Scale bars: 5 mm (low magnification) and 50 μm (high magnification). Red letter T and circles indicate the tumor area in the pancreas.(L) Quantification of CD8A IHC staining. Each color within a group represents an individual mouse. Four non-overlapping regions from the same section were analyzed and shown in the same color. Sample sizes: EC, n = 7; HSR, n = 8. Wilcoxon test.