Fig 1: 1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3) imposes a gene signature in human colon cancer-associated fibroblasts (CAFs) that is associated with longer survival of patients with colorectal cancer (CRC). (A) Kaplan–Meier survival curves showing the impact of the 1,25(OH)2D3-associated gene signature on the disease-free survival (DFS) of patients with CRC from three unrelated publicly available external data sets (GSE33113, n=89, American Joint Committee on Cancer (AJCC) stage II; GSE14333, n=226, AJCC stage I–II–III; GSE39582, n=497, AJCC stage I–II–III). (B) Kaplan–Meier survival curves showing the impact of the 1,25(OH)2D3-associated gene signature on the overall survival (OS) of patients with CRC from GSE39582 data set (n=562, AJCC stage I–II–III–IV). (C) Representative immunohistochemical images of CD82 and S100A4 protein expression in human CRC samples. Bars, 20 μm. (D) Box plots showing CD82 or S100A4 protein expression in stromal fibroblasts located in colorectal tumours with low or high vitamin D receptor (VDR) protein expression levels in stromal fibroblasts (n=658). (E) Kaplan–Meier survival curves depicting OS of patients with CRC (n=658) stratified by CD82 or S100A4 protein expression levels in tumour stromal fibroblasts.