Fig 2: CCN2 scoring of megakaryocytes. Megakaryocytes stained for CCN2 demonstrating a no staining (score: 0), b weak staining (score: 1 +), c moderate staining (score: 2 +), and d strong staining (score: 3 +)

Fig 3: CCN2 immunohistochemical staining of bone marrow biopsies. a Normal bone marrow, in which megakaryocytes show no or only weak staining, with only rare megakaryocytes displaying moderate staining. Granulopoiesis and erythropoiesis are negative. b An example of a myeloproliferative neoplasm displaying marked overexpression of CCN2 with many megakaryocytes showing moderate to strong cytoplasmic staining

Fig 4: SKIL induced autophagy through inhibition on TAZ phosphorylation by LATS2 and degradation.a Western blot analysis showed that SKIL silencing decreased expression levels of autophagy markers (LC3, p62, and Beclin-1). b, c Immunofluorescence staining indicated significant decrease of autophagosome after SKIL silencing in both CALU-3 and NCI-H520 cell lines. Percentage of cells with LC3 vacuoles were counted and calculated from 100 cells in 20 fields d Western blot analysis showed that SKIL silencing decreased expression of TAZ and its downstream signaling factors (CTGF, CYR61). e, f Cycloheximide treatment resulted in faster TAZ protein degradation in SKIL-silenced CALU-3 (shSKIL) compared to control (shNC). g Co-immunoprecipitation assay showed that SKIL could bind to elements of Hippo complex (LATS2, Sav), but not TAZ. h Western blot analysis showed that overexpression of LATS2 led to increased phosphorylation of TAZ (p-TAZ) and decreased levels of TAZ. Further overexpression of SKIL reversed the effect of LATS2 overexpression on levels of p-TAZ and TAZ, without influencing LATS2 level. *P < 0.05, **P < 0.01. Experiments were performed in triplicate.