Fig 1: Effect of ALDH1L1 on enzymes of carnitine metabolism(A) Schematic presentation of acylcarnitine metabolism; the nature of the link between folate and carnitine metabolism is not clear. (B) Protein expression and (C) quantification of band intensities of CPT1A and CPT2 in A549 cells (immunoblot assay) at different time points post-transduction of ALDH1L1. (D) Analysis of carnitine enzyme mRNA expression (qPCR) in A549 72 h post-transduction of ALDH1L1. CPT1A, carnitine palmitoyltransferase 1A; CPT1B, carnitine palmitoyltransferase 1B; CPT1C, carnitine palmitoyltransferase 1C; (E) CPT2, carnitine palmitoyltransferase 2; CROT, carnitine o-octanoyltransferase; CRAT, carnitine acetyltransferase; CACT, mitochondrial carnitine-acylcarnitine-like translocase. Data are presented as the mean of three replicates ± SEM. Non-significant differences are denoted as “NS.” Uncropped and unmodified images of the immunoblots shown in (B) are presented in Figure S13.