Fig 1: Upstream transcriptional regulator of FNDC4. A, Venn diagram showing the overlap of five datasets and workflow identifying BHLHE40. DEG, differentially expressed genes. B, ChIP-qPCR for KLF6, PPARG (top), and BHLHE40 (bottom) binding to the FNDC4 upstream regulatory region [−1 kb from transcription start site (TSS)]. C, qRT-PCR for BHLHE40 and FNDC4 mRNA in SiC002 PDAC cells with or without EMT-inducing MCM treatment. D, Western blot analysis for BHLHE40 and FNDC4 proteins following MCM treatment in two primary PDAC models. E and F, qRT-PCR (E) and Western blot analysis (F) for BHLHE40 and FNDC4 expression after oncostatin M (OSM) treatment in two PDAC models. G and H, qRT-PCR in SiC002 cells (G) and Western blot analysis in SiC002 and SiC005 (H) for BHLHE40 and FNDC4 following BHLHE40 knockdown. I, Quantification of the invasion assay in SiC002 cells following BHLHE40 knockdown. J and K, qRT-PCR in SiC002 cells (J) and Western blot analysis in SiC002 and SiC005 cells (K) for BHLHE40 and FNDC4 after MCM or TGFβ treatment, alone or combined with TGFβ antibodies or siBHLHE40. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.