Fig 1: eIF3D and RBPs cooperate to regulate mRNA translation following mTOR inhibition(A) MEFs stably expressing HA-eIF3D proteins were treated with/without Torin1 (250 nM) for 24 h, and RNA immunoprecipitation and qRT-PCR were performed. Data are the mean ± SD of three technical replicates. Statistical significance (*p < 0.05) was assessed by t test.(B) eIF3D interacting RBPs identified by mass spectrometry.(C–E) MEFs stably expressing control, HA-hnRNPF (C), HA-hnRNPK (D), and HA-SSB (E) were treated with Torin1 for 24 h, and their interacting proteins were identified and analyzed.(F–K) Stably knocked down MEFs with indicated shRNAs were treated with/without Torin1 for 24 h, and immunoblot blot analysis was performed.(L) RBP knockdown MEFs expressing HA-eIF3D were treated with Torin1 for 24 h, and RNA immunoprecipitation and qRT-PCR were performed. Data are the mean ± SD of three technical replicates. Statistical significance (*p < 0.05) was assessed by t test.(M) Stably knocked-down MEFs with indicated shRNAs were treated with/without Torin1 for 24 h, and qRT-PCR was performed. Data are the mean ± SD of three technical replicates. Statistical significance (*p < 0.05) was assessed by t test.See also Figure S6.