Fig 2: Downregulation of TROP2 inhibits cell proliferation(a) Cell viability was determined by CCK-8 assay following transfection with TROP2-siRNA sequence (40 pmol/mL) for 24 hours in HepG2 cells. (b) Cell viability was determined by CCK-8 assay following transfection with TROP2-siRNA sequence (40 pmol/mL) for 24 hours in HCCLM3 cells. The results are shown as mean ± standard deviation. **p < 0.01 vs. BC group.

Fig 3: Curcumin suppresses the expression of Trop2. (A) Western blot analysis of curcumin-treated bladder cancer cells showed that Trop2 was an important target of curcumin. (B) Quantitative results of the western blot analysis. *P<0.05 and **P<0.01, vs. control. Trop2, trophoblast cell surface antigen 2.

Fig 4: Downregulation of Trop2 enhances curcumin-mediated inhibition of Trop2. (A) Expression of Trop2, p27 and cyclin E1 was detected following Trop2 silencing in bladder cancer cells. (B) Quantitative results of expression. *P<0.05, vs. control; #P<0.05, vs. 15 µM curcumin treatment or Trop2 siRNA transfection. Trop2, trophoblast cell surface antigen 2; siRNA, small interfering RNA.

Fig 5: Associations between CORO1C, TROP2, and CRC metastasis. Overexpression of Trop2 in 293T cells was confirmed by (A) qRT-PCR, (B) Western blotting, and (C) Immunofluorescence. (D) Trop2 immunocomplex by Co-IP; (E) Heatmap of proteins interacting with Trop2 in PC and MC. The RNA-seq data were obtained from GSE28702. Red and green colors represent high and low gene expression, respectively. PC: primary CRC; MC: metastatic CRC, **p < 0.01.