Fig 1: MT3R activation during sleep reduces protein kinase activity and dephosphorylates synaptic proteins. a) Immunoblot analysis of protein kinase phosphorylation levels after chemogenetic inhibition neurons in the superficial layer of the MEC during SD. p‐PKA (mean ± SEM), Student's t‐test, **P < 0.01, n = 8 rats for each group; p‐PKC (mean ± SEM), Student's t‐test, ***P < 0.001, n = 8 rats for each group. b) Immunoblot analysis of cytoskeleton co‐factors after chemogenetic inhibition neurons in the superficial layer of the MEC during SD. Rac1/Cdc42 (mean ± SEM), Welch's t‐test, **P < 0.01, n = 8 rats for each group; AKAP5 (mean ± SEM), Student's t‐test, *P < 0.05, n = 8 rats for each group. c) Immunoblot analysis of synaptic proteins phosphorylation levels after chemogenetic inhibition neurons in the superficial layer of the MEC during SD. p‐synapsin‐1 (mean ± SEM), Student's t‐test, *P < 0.05, n = 8 rats for each group; p‐PSD95 (mean ± SEM), Student's t‐test, *P < 0.05, n = 8 rats for each group. d) Immunoblot analysis of glutamate receptor subunit phosphorylation levels after chemogenetic inhibition neurons in the superficial layer of the MEC during SD. p‐GlutR1 (mean ± SEM), Student's t‐test, **P < 0.01, n = 8 rats for each group; p‐GlutR2 (mean ± SEM), One‐Sample t‐test, *P < 0.05, n = 8 rats for each group. e) The paradigm for superficial layers of the MEC collection after drug microinfusion during sleep. MEC, medial entorhinal cortex. Scale bar: 500 µm. f) Immunoblot analysis of protein kinase phosphorylation levels after administration of saline and S29434. p‐PKA (mean ± SEM), Student's t‐test, **P < 0.01, n = 8 rats for each group; p‐PKC (mean ± SEM), Welch's t‐test *P < 0.05, n = 8 rats for each group. g) Immunoblot analysis of cytoskeleton co‐factors after administration of saline and S29434. Rac1/Cdc42 (mean ± SEM), Student's t‐test, *P < 0.05, n = 8 rats for each group; AKAP5 (mean ± SEM), Student's t‐test, *P < 0.05, n = 8 rats for each group. h) Immunoblot analysis of synaptic protein phosphorylation levels after administration of saline and S29434. p‐synapsin‐1 (mean ± SEM), Student's t‐test, *P < 0.05, n = 8 rats for each group; p‐PSD95 (mean ± SEM), Mann‐Whitney Rank Sum Test, *P < 0.05, n = 8 rats for each group. i) Immunoblot analysis of glutamate receptor subunit phosphorylation levels after administration of saline and S29434. p‐GlutR1 (mean ± SEM), Student's t‐test, *P < 0.05, n = 8 rats for each group; p‐GlutR2 (mean ± SEM), Student's t‐test, *P < 0.05, n = 8 rats for each group. j) Immunoblot analysis of actin‐binding protein Profilin and cytoskeleton after administration of saline and S29434. profilin (mean ± SEM), Welch's t‐test, *P < 0.05, n = 8 rats for each group; F/G actin ratio (mean ± SEM), Welch's t‐test, **P < 0.01, n = 8 rats for each group. To standardize, the mean of the relative expression abundance (target protein grayscale value/GAPDH grayscale value) of each group's control was set as 1. p‐PKA, phosphorylation of Protein Kinase A; p‐PKC, phosphorylation of Protein Kinase C; AKAP5, A‐Kinase Anchor Protein 5; p‐PSD95, phosphorylation of Postsynaptic Density protein‐95.