Fig 1: Maximal EGF-induced RON phosphorylation requires kinase activity of both receptors.(A and B) A431RON cells were pre-treated with 10 μM afatinib (Afat, pan-ErbB inhibitor) or 1 μM BMS777607 (BMS, Met family kinase inhibitor) for 20 or 15 min, respectively. Cells were then treated ± EGF or MSP for 5 min. (A) Cell lysates were used for PY1068 and EGFR immunoblots. (B) Lysates were immunoprecipitated (IP) with an anti-HA antibody and then immunoblotted for PY and RON. All samples are from the same blot, but an extraneous lane was removed for clarity. Bar graphs are corresponding mean ± SD from triplicate biological experiments. * p < 0.05; ** p < 0.01; *** p < 0.001. Figure 5—source data 1.Full raw western blots and blots with relevant bands labelled, corresponding to Figure 5A,B. Figure 5—source data 2.Source data for quantification of blots in Figure 5A,B.