Fig 2: Western blot quantification of cytosolic and nuclear protein levels of Ikk and NF-kB subunits in adult male mice with hepatocyte-specific activation of Ikk2 (Liv-Ikk2ca). a cytosol; b nuclei. The densities of Ikk and each NF-kB subunit in the gel image (top) were normalized to Gapdh (a) or histone H3 (b). N = 3 per group, mean ± SE. *p < 0.05 versus wild-type mice

Fig 3: Hepatic mRNA expression of key antioxidative genes and Nqo1 activities in adult male mice with hepatocyte-specific activation of Ikk2 (Liv-Ikk2ca). a mRNA expression of antioxidative genes; and b Nqo1 activities. N = 6 per group, mean ± SE. *p < 0.05 versus wild-type mice

Fig 4: Diagram that summarizes the possible underlying mechanisms of key changes in gene expression and pathophysiology in adult male mice with hepatocyte-specific activation of Ikk2 (Liv-Ikk2ca). Genes that are active or induced in Liv-Ikk2ca mice are in green shape, whereas genes that are inactive or unchanged in Liv-Ikk2ca mice are in red shape. Pathways/responses that are activated in Liv-Ikk2ca mice are marked with (+), whereas pathways/responses that are inhibited or unchanged in Liv-Ikk2ca mice are marked with (−)

Fig 5: Hepatic expression of genes important in metabolism of glucose and lipids as well as cholesterol and bile acids in adult male mice with hepatocyte-specific activation of Ikk2 (Liv-Ikk2ca). a mRNA expression of genes in glucose and lipid metabolism; b mRNA expression of genes in cholesterol and bile acid metabolism. N = 6 per group, mean ± SE. *p < 0.05 versus wild-type mice. c & d Hepatic protein levels of Scd1 in adult male Liv-Ikk2ca mice. The densities of Scd1 in the gel image (top) were normalized to Gapdh (bottom). N = 3 per group, mean ± SE