Fig 2: Two SCLC cell lines (Lu-135 and STC-1) express L1CAM. (A) Phase contrast images of Lu-135 and STC-1 cells with L1CAM silencing. Cells were reverse-transfected with NC siRNA, L1CAM siRNA #1, or L1CAM siRNA #2 (10 nM each) and cultured for 48 h. Scale bars: 200 μm (left; low magnification) and 50 μm (right; high magnification). (B) Conventional RT-PCR for the expression of L1CAM, SYP, NEUROD1, and ACTB mRNA in Lu-135 and STC-1 cells. Cells were treated as described in (A). (C) Western blot analysis of the cells treated as described in (A). Of note, NCI-H69 was used as a positive control for ASCL1. (D) Western blot analysis of HuL cells and SCLC-N cells for L1CAM. (E) Correlation between L1CAM and NEUROD1 or ASCL1 mRNA expression in SCLC cell lines (n = 52). (F) Regulated Gene Ontology results for the top 100 protein-coding DEGs in L1CAM-silenced Lu-135 cells compared with control cells.