Fig 2: Senataxin (SETX) and RANBP3 localization in meiotic spreads after ATR inhibition.(A) Immunofluorescence of meiotic chromosome spreads with SETX (green) and SYCP3 (red) from mice collected 4 hr after 50 mg/kg treatment with AZ20 or vehicle. (B) Quantification of pachytene spreads in a (four vehicle mice; n = 237 cells; four ATRi mice; n = 283 cells p=0.00435 measured by Student’s t-test). (C) Rad1 cKO and control spreads stained as in (A). (D) Quantification of pachytene or pachytene-like spreads in (C) (four control mice, n = 64 cells; four RAD1 cKO mice, n = 72 cells p=0.00286 measured by Student’s t-test). (E) Immunofluorescence of meiotic chromosome spreads with RANBP3 (green) and SYCP3 (red) from mice collected 4 hr after 50 mg/kg treatment with AZ20 or vehicle. (F) Quantification of pachytene spreads in e (three vehicle mice, n = 174 cells; three ATRi mice, n = 167 cells p=0.048 measured by Student’s t-test). (G) Rad1 cKO and control spreads stained as in (E). (H) Quantification of pachytene or pachytene-like spreads in (G) (four control mice, n = 96 cells; four cKO mice, n = 99 cells p=0.0039 measured by Student’s t-test).

Fig 3: Effect of ATR inhibition on RANBP3 localization in meiotic spreads.(A) Quantification of RANBP3 intensity from meiotic spreads separated by animal. (B) Example spreads from mice collected 4 hr after 50 mg/kg treatment with AZ20 or vehicle. (C) Quantification of RANBP3 at the sex body or sex chromosomes body of control or Rad1 cKO meiotic spreads, respectively. (D) Example images. See Materials and methods for more details on quantification. Data derived from 8- to 12-week-old animals, except when noted by postnatal day 14 (PND14).