Fig 2: The percentages of CD4+CD25+CD127+ (inactivated T regulatory cells) in peripheral blood mononuclear cells (PBMNCs) compared between PBMNCs cultured in Quality and Quantity culture media (QQ-media) and PBMNCs cultured in standard culture media. All experiments were performed in triplicate. Forward and side scatter plots were used to gate the lymphocyte populations. The gated cells were then evaluated to identify CD3+CD4+ cells. The CD3+CD4+ cells were then gated to assess the populations of CD4+CD25+and CD127+ cells. Student's t-test was used for phenotypic analysis

Fig 3: Increased numbers of ILCs in blood of psoriatic patients with metabolic disorders. (A) Gating strategy to define subpopulations of innate lymphoid cells ILC1, ILC2, ILC3. Analysis was performed by flow cytometry by using lineage Cocktail-Pacific Blue (Biolegend), CD11c-BV421, CD34-BV421, CD94-BV421, FcERIa-BV421, CD45-VioGreen, CD117-PE (c-Kit), IL7RA-APC (CD127), CRTH2-APC-Cy7 (CD294). ILCs are pregated as CD45 positive cells that are further defined by negative expression of the lineage cocktail and positive staining for IL7RA ( Supplementary Figure 2 ). The figure is presenting cells covering these criteria and their further subdivision by individual expression of surface antigens CD117 and CRTH2. ILC1 are blue bordered (CD117-, CRTH2-), ILC2 red (CRTH2+) and ILC3 orange (CD117+, CRTH2-). Total ILCs are colored in green. (B) Number of ILCs of healthy controls (unfilled dots) and patients with psoriasis vulgaris (filled dots) in blood, mean and SD, each dot represents an individual patient, Mann-Whitney test. (C) Number of ILCs in blood of normal-weight psoriatic patients (body mass index, BMI<30 kg/m2; unfilled triangle; n=8) and obese patients (BMI =30 kg/m2; filled triangle; n=8) are shown, mean and SD, Mann-Whitney test. (D) Number of ILCs in blood of psoriatic patients without atherosclerosis (unfilled trapezoid; n= 9) and with atherosclerosis (filled trapezoid; n=7) are shown, mean and SD, Mann-Whitney test. (E) Number of ILCs of psoriatic patients without hepatic remodeling (cross symbol; n=7) and patients with steatosis hepatis (star; n=9), mean and SD, Mann-Whitney test. (F) Linear correlation of the number of circulating ILC and BMI (n=15), Pearson correlation. (G) Linear correlation of the number of circulating ILC1 and PROCAM score [%] (n=16), Pearson correlation. *p < 0.05; **p < 0.01; ***p < 0.001. ns, not significant.

Fig 4: T cell phenotype and senescence/exhaustion markers in MDD(A–D) PBMCs from patients with MDD and matched HC were examined by flow cytometry. CD4+ (upper panel) and CD8+ T cell subpopulations (lower panel) were analyzed for the expression of markers classifying differentiation (CD45RA, CCR7; n = 25) (A), chemokine receptors (CXCR3, CCR6, CCR4; n = 25) or regulatory T cell markers (CD25, CD127; n = 22) (B), markers indicative of exhaustion or senescence (PD-1, KLRG1, CD57; n = 19–20) (C) or inhibitory receptors (CTLA-4, LAG-3, Tim-3; n = 20) (D). Naive/Memory subpopulations were defined as TNaive (CCR7+/CD45RA+), TEM (CCR7-/CD45RA-), TCM (CCR7+/CD45RA-), and TEMRA (CCR7-/CD45RA+). T helper cell subsets were defined as Th1 (CXCR3+/CCR6-), Th2 (CXCR3-/CCR6-/CCR4+), Th17 (CXCR3-/CCR6+/CCR4+), and Th1/Th17 cells (CXCR3+/CCR6+) (n = 25). For the analysis of inhibitory receptors, PBMCs were stimulated with 10 µg/mL of a-CD3 and 1 µg/mL a-CD28 for 48 h. Median and interquartile ranges are shown. Wilcoxon signed-rank test was used to compare groups. All p values > 0.1 if not otherwise indicated.

Fig 5: Innate Lymphoid Cell (ILC) phenotypes in peripheral blood mononuclear cells of 17 HIV infected cART-treated and 17 age-matched healthy HIV –negative adults. ILC precursor cells were considered as lineage –(CD3-,CD19-,CD14-,CD29-, CD56-lymphocytes), CD127 + and CD117 + cells. ILC1 cells were determined by their expression of T-BET, CD161 and lack of expression of CRTH2. ILC2 cells were determined by their expression of CD161 + and CRTH2. ILC3 cells were determined by their expression of CD161 + and ROR?t