ZO-1 (D7D12) Rabbit mAb 8193S from Cell Signaling Technology

Product Specs
ItemZO-1 (D7D12) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number8193S
Quantity100 µl
ApplicationsW, IP
ReactivityH, Mk
TargetZO-1
HostRabbit
NCBI Gene AliasesZO-1
ClonalityMonoclonal
NCBI Full Gene Nametight junction protein 1
Research AreaCytoskeletal Signaling
IsotypeIgG
CloneD7D12
Molecular Weight220

Questions and Answers

Can this antibody be used to study epithelial-to-mesenchymal transition in cancer cells?

Yes, the ZO-1 (D7D12) Rabbit mAb has been utilized in studies investigating epithelial-to-mesenchymal transition (EMT). For instance, it was shown to be effective in suppressing fibronectin-induced EMT in breast epithelial cells, where its expression was analyzed alongside other EMT markers such as E-cadherin and N-cadherin ().

What applications has this antibody been shown to work in?

The ZO-1 (D7D12) Rabbit mAb is suitable for use in Western Blotting (WB) and Immunoprecipitation (IP). It has been cited in various studies, including those examining the role of ZO-1 in maintaining tight junction integrity and its involvement in signaling pathways related to cancer progression ().

Is this antibody effective in non-human primate models?

Yes, the ZO-1 (D7D12) Rabbit mAb has been shown to react with proteins in non-human primates, as well as humans, making it applicable for comparative studies in both species ().

How does this antibody contribute to understanding cancer biology?

The ZO-1 (D7D12) Rabbit mAb has been used to elucidate the role of tight junction proteins in cancer biology, particularly in studies that link ZO-1 expression to tumor suppression and the regulation of cell proliferation and invasion in various cancer types ().

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Bacteroides finegoldii and Parabacteroides goldsteinii Mediate Fucoidan-Induced Attenuation of Intestinal Inflammation in Mice Through Betaine- and Spermidine-Related PathwaysFoodsJanuary 7, 2026Tao Qin, Yifan Wei, Weiyun Zheng, Shugang Li, Shuang Song, Chunqing Ai(38519), p65 (8242T), p-IκBα (2859T), p-p38 (8690), p-p65 (3033T), p38 (4511), p-MLC2 (3671T), ZO-1 (8193), and claudin-1 (4933) (CST, Danvers, MA, USA), and anti-occludin (ab216327), MLCK (ab232949), MLC2

(2) Ovarian cancer metastasis to the human omentum disrupts organ homeostasis and induces fundamental tissue reprogrammingNature CommunicationsDecember 16, 2025Ulrike Lischetti, Ching-Yeu Liang, Matteo Carrara, Ricardo Coelho, Antoine Hanns, Flavio Lombardo, Arthur Dondi, Sandra Goetze, Michal Hensler, Christian Kurzeder, et al.anti-CD200 (Cell Signaling Technology, cat. no. 23451) and anti-ZO-1 (Cell signaling Technology, cat. no. 8193) antibodies. For ascites exposure experiment, three primary mesothelial populations isolated from three

(3) RepID promotes metastatic potential in osteosarcoma through regulation of the PRC1-GATA6 axisAnim Cells Syst (Seoul)December 8, 2025Dong-Hyun Jung, Jong-Uk Park, Jae-Hyun Jo, Dong-Kyu Kim, Yeong-Mu Kim, Min-Ju Kang, Suk-Hoon Hong, Hyo Je Cho, Jihoon Nah, Jun-Young Park, et al.(Cell Signaling, 9585, 1:2,000), anti-VIM (Cell Signaling, 5741, 1:2,000), anti-ZO-1 (Cell Signaling, 8193, 1:2,000), anti-H3K4me2 (Millipore, 07-030, 1:5,000), anti-H3K4me3 (Millipore, 05-1339, 1:5,000), anti-H3K9me2

(4) α-Parvin regulation of cell re-arrangement is critical for ureteric bud branching morphogenesisbioRxivDecember 8, 2025Xinyu Dong, Fabian Bock, Ali Hashmi, Nada Bulus, Glenda Mernaugh, Gema Bolas, Shensen Li, Wanying Zhu, Meiling Melzer, Kyle Brown, et al.(D7F9),Cell Signaling,8190 Mouse anti-α-Parvin (clone 1D4),MilliporeSigma,MABT1441 ZO-1,Cell Signaling,8193 Rabbit monoclonal anti-E-cadherin (24E10),Cell Signaling,3195 Purified mouse E-cadherin (clone 36),BD Bioscience

(5) Establishment and comprehensive characterization of a subline with highly bone-metastatic propensity derived from the lung adenocarcinoma A549 cell lineJournal of Bone OncologyDecember 1, 2025Yujian Xu, Yahan Qin, Wenjun Chai, Ke Xue, Xiaoli Liu, Jing Li, Yue Cao, Lei Sun, Hongyu Pan, Mingxia Yanused in this study were as follows: anti-N-Cadherin(13116, CST), anti-E-Cadherin(3195, CST), anti-ZO-1(8193, CST), anti-ZEB1(3396, CST), anti-Vimentin(5741, CST), anti-Claudin-1(13255, CST), anti-β-Catenin(8480,

(6) Gut microbiota involvement in the alteration of inflammatory cell infiltration and gut barrier integrity in liver cirrhosisBiomedical ReportsOctober 20, 2025Kaiduan Xie, Yiwang Zhang, Shuyan Tan, Jiajie Luo, Xingtong Ou, Siwei Tanactin (cat. no. ab5694; both Abcam), -claudin-1 (cat. no. 13255S), -zonula occludens-1 (ZO-1; cat. no. 8193T) and -E-cadherin (cat. no. 14472S; all Cell Signaling Technology, Inc.). ELISA DAO and endotoxin levels

(7) Inhibition of NR2F2 suppresses invasion ability and modulates EMT marker in head and neck squamous cell carcinomaDiscov OncolOctober 15, 2025Joo Kyung Noh, Seon Rang Woo, Min Kyeong Lee, Jung Woo Lee, Young Chan Lee, Seong-Gyu Ko, Young-Gyu Eunanti-NR2F2 (6434 S, Cell Signaling Technology), anti-actin (sc-47778, Santa Cruz Biotechnology), anti-ZO-1 (8193 S, Cell Signaling Technology), anti-claudin-1 (13255 S, Cell Signaling Technology), anti-N-cadherin

(8) Thyroid Hormone T4 Alleviates Traumatic Brain Injury by Enhancing Blood-Brain Barrier IntegrityInt J Mol SciOctober 3, 2025Mayuri Khandelwal, Zhe Ying, Fernando Gomez-PinillaUSA)",sc-293072,95 pVEGFR2,Cell signaling,3770,230 VEGFR2,Cell signaling,9698,210 and 230 ZO-1,Cell signaling,8193,220 OXPHOS,"Invitrogen (Carlsbad, CA, USA)",45-8099,"55, 48, 40, 30, and 20" Iba-1,"Fujifilm Wako

(9) Roles of protein–protein interactions and monolayer mechanics in tricellulin localization to tricellular tight junctionsBiology OpenSeptember 15, 2025Toiba Mushtaq, Jaakko Lehtimäki, Konstantin Kogan, Johan Peränen, Xionan Liu, Markku Varjosalo, Aki Manninen, Pekka Lappalainen(WB) and immunofluorescence (IF) microscopy: rabbit monoclonal anti-ZO1 (Cell Signaling Technology, 8193S, 1:100 for IF), rabbit monoclonal anti-LSR (Atlas, HPA007270, 1:150 for IF, 1:500 for WB), rabbit polyclonal

(10) KSHV and HPV modulate epithelial-to-mesenchymal transition in oral epithelial cellsmBioSeptember 10, 2025Qian Li, Sharon E. Hopcraft, Philip T. Lange, Linda Pluta, Dirk P. Dittmer, Cary A. Moody, Blossom DamaniaE-cadherin (Invitrogen, 33-4000), vimentin (Proteintech, 10366-1-AP), ZO-1 (Cell Signaling Technology, 8193T), beta-catenin (Cell Signaling Technology, 8480T), Slug (Cell Signaling Technology, 9585T), GFP (Abcam,

(11) A fetal oncogene NUAK2 is an emerging therapeutic target in glioblastomaEMBO molecular medicineSeptember 1, 2025Jo H, Munoz S, Dalvi A, Yang W, Morozova E, Glasgow SM.,Thermo Fisher Scientific,C10639 Anti-EFTUD2,Novus,NBP1-87019 anti-GAPDH,Millipore,MAB374 Anti-HSP90,stressMarq,SMC-137 anti-Ki67,CST,12202S anti-N-Cadherin,CST,13116 anti-NUAK1,CST,4458S anti-NUAK2,Abcam,ab224079 Anti-PCNA,Sigma,MAB424 Anti-MYPT1,ProteinTech,22117-1-AP Anti-YAP1,CST,14074 Anti-TAZ,CST,4883 Anti-LATS1,CST,C66B5 anti-Slug,CST,9585 anti-Snail,CST,3879 Anti-Stem121,Takara,Y40410 anti-Vimentin,CST,5741 anti-ZEB1,CST,3396 anti-ZO-1,CST,8193 anti-β-Catenin,CST,8480 Anti-Stem121,Takara,Y40410 Cell cycle regulation sampler antibody kit,CST,9932 Hoechst

(12) TFAP2A enhances tumor stemness and promotes metastasis in pancreatic ductal adenocarcinomaiScienceAugust 15, 2025Jiaxin Luo, Zezhi Ding, Dongjie Chen, Yongsheng Jiang, Yizhi Cao, Minmin Shi, Xiaomei Tang, Jia Liu, Meilin Xue, Zehui Zhang, et al.RRID:AB_2291471 Anti-Claudin-1,Cell Signaling Technology,Cat#13255; RRID:AB_2798163 Anti-ZO-1,Cell Signaling Technology,Cat#8193; RRID:AB_10898025 Anti-Vimentin,Cell Signaling Technology,Cat#5741; RRID:AB_10695459 Anti-Snail,Cell Signaling Technology

(13) Maternal Western diet increases inflammatory markers and decreases barrier function of offspring in Papio anubisAmerican Journal of Physiology-Gastrointestinal and Liver PhysiologyAugust 1, 2025Grant H. Gershner, Alena Golubkova, Cody Dalton, Camille Schlegel, Chase Calkins, Darlene N. Reuter, Megan Learner, James F. Papin, Sunam Gurung, Karen R. Jonscher, et al.3195, Cell Signaling, Danvers, MA), zona occludins 1 (ZO-1, D7D12) rabbit mAB (1:200 dilution, Cat. No. 8193, Danvers, MA), or NF-κB p65 (ab16502) rabbit mAB (1:1,000 dilution, Abcam, Cambridge, MA). Following

(14) Blocking YAP1-Liprin-β2 interaction impedes metastasis and promotes tumor suppression in head and neck squamous carcinomaSci RepJuly 24, 2025Seon Rang Woo, Joo Kyung Noh, Min Kyeong Lee, Moonkyoo Kong, Soonki Min, Ji-Youn Sung, Kiyong Na, Suyeon Kang, Junho K. Hur, Seong-Gyu Ko, et al.Cell Sinaling Technology, MA, USA), anti-E-cadherin (3195S, Cell Signaling Technology), anti-ZO-1 (8193S, Cell Signaling Technology), anti-N-cadherin (13116S, Cell Signaling Technology), and anti-GAPDH (sc-365062,

(15) Lumefantrine ameliorates DSS-induced colitis by targeting FLI-1 to suppress NF-κB signalingFront PharmacolJuly 11, 2025Ji Yang, Peng Guo, Hongtao Luo, Xin Tang, Wei Liu, Xiaolin RenThe primary antibodies used were: Claudin-1 (Abcam, ab15098, Cambridge, United Kingdom), ZO-1 (CST, 8193P, Massachusetts, United States), p-p65 (Proteintech, 82335-1-RR, Wuhan, China), p65 (CST, 8242S, Massachusetts,

(16) Utilization of artificial circular RNAs as miRNA sponges and anti-PD-1 scFv expression platforms to suppress hepatocellular carcinoma progressionFront ImmunolJune 11, 2025Yongping Lai, Fei Wang, Guang Cai, Yingying Li, Jiaxin Weng, Feifan Cai, Leijie Cai, Niangmei Cheng, Bixing Zhao, Yongyi ZengBiorbyt), anti-snail (1:2000, CST, #3879), anti-vimentin (1:2000, CST, 5741s), anti-ZO-1 (1:2000, CST, 8193P), and anti-VEGFR (1:1000, proteintech, 13687-1-AP). Detection was performed using enhanced chemiluminescence

(17) The role of gut leakage and immune cell miss-homing on gut dysbiosis-induced lung inflammation in a DSS mice modelPLoS OneMay 28, 2025Mariam Wed Eladham, Narjes Saheb Sharif-Askari, Priyadharshini Sekar, Bushra Mdkhana, Balachandar Selvakumar, Baraa Khalid Salah Al-Sheakly, Fatemeh Saheb Sharif-Askari, Ibrahim Hachim, Rabih Halwani5% BSA prepared in Tris-Buffered Tween saline for 1hr and incubated with primary anti- ZO-1 (cat. #8193, 1:1000), anti- Claudin1 (cat. #13255, 1:1000), anti- Occludin1 (cat. #91131, 1:1000) or anti-βactin

(18) Ubiquitin specific protease 7 is a potential therapeutic target for gastric cancerFrontiers in OncologyFebruary 21, 2025Zhi-Ru Wang, Wen-Ting Kang, Rui-Li Yu(Abcam No,21624), E-cadherin (Cell Signaling Technology No. 3195S), ZO1 (Cell Signaling Technology No. 8193S), Vimentin (Cell Signaling Technology No. 5741S). 2.3 Immunohistochemical analysis Gastric carcinoma

(19) Mechanism of β‑sitosterol in treating keloids: Network pharmacology, molecular docking and experimental verificationMol Med RepFebruary 14, 2025Pingping Huo, Zhouna Li, Shan Jin, Sujie Wang, Yinli Luo, Lianhua Zhu, Zhehu JinLtd.), anti-Snail (3879; 1:1,000; CST Biological Reagents Co., Ltd.), anti-zonula occludens-1 (ZO-1; 8193; 1:1,000; CST Biological Reagents Co., Ltd.) and anti-β-actin (4967; 1:1,000; CST Biological Reagents

(20) Establishment and characterization of three gemcitabine-resistant human intrahepatic cholangiocarcinoma cell linesSci RepFebruary 9, 2025Jiandong Li, Yanxin Hu, Jiayao Zhang, Weiguang Zhang, Jianhua Yu, Baochun Lu(#3195), N-Cadherin (#13116), β-catenin (#8480), Vimentin (#5741), Snail (#3879), ZEB1 (#3396), ZO-1 (#8193), Bcl-2 (#15071), Bax (#2772), and β-actin (#3700) were procured from Cell Signaling Technology (Boston,

Be the First to Write a Review!

ZO-1 (D7D12) Rabbit mAb

ZO-1 (D7D12) Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (169)

Reviews

ZO-1 (D7D12) Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map