Fig 1: (A–D) BRCA2 and PALB2 facilitate ssDNA formation and homologous recombination at telomeres in FANCM deficient ALT cells. siRNA transfected U2-OS cells were co-stained with antibodies recognizing BLM and TRF1, or pRPA and TRF1, or Rad51 and TRF1. (E–G) FANCD2 is recruited to the telomeres in FANCM deficient cells and facilitates the recruitment of BLM. siRNA transfected U2-OS cells were co-stained with antibodies recognizing FANCD2 and TRF1, or BLM and TRF1. More than three co-localized FANCD2 and TRF1 foci were counted. All error bars are standard deviation of the mean obtained from three different experiments. Standard two-tailed Student’s t-test: *p < 0.05, **p < 0.01, ***p < 0.001, ns – not significant.