Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).