ASCT2 (D7C12) Rabbit mAb 8057S from Cell Signaling Technology

Product Specs
ItemASCT2 (D7C12) Rabbit mAb
CompanyCell Signaling Technology
Price $399.00 Inquire
Catalog Number8057S
Quantity100 µl
ApplicationsW, IP, IHC-P, IF-IC, FC-FP
ReactivityH, Mk
TargetASCT2
HostRabbit
NCBI Gene AliasesAAAT, ASCT2, ATBO, M7V1, M7VS1, R16, RDRC
ClonalityMonoclonal
NCBI Full Gene Namesolute carrier family 1 member 5
Research AreaMetabolism
CloneD7C12
Molecular Weight49, 75

Questions and Answers

Can this antibody be used to study glutamine metabolism in cancer cells?

Yes, the ASCT2 (D7C12) Rabbit mAb has been utilized in studies investigating glutamine metabolism in cancer cells. For instance, it was employed to assess the expression of the SLC1A5 transporter, which is crucial for glutamine uptake in various cancer types, including mesothelioma and breast cancer, as demonstrated in multiple publications.

Is this antibody effective for Western blot analysis?

The ASCT2 (D7C12) Rabbit mAb has been shown to work effectively in Western blot applications, as evidenced by its use in studies where protein extracts were analyzed to determine ASCT2 levels in different cell lines and tissues, including those related to cancer research.

What species can this antibody detect?

This antibody recognizes endogenous levels of total ASCT2 protein in human and non-human primate species, making it suitable for studies involving these organisms.

Has this antibody been cited in any significant research studies?

Yes, the ASCT2 (D7C12) Rabbit mAb has been cited in 45 scientific articles, indicating its relevance and utility in various research contexts, particularly in studies related to metabolism and cancer biology.

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Glutamine transporter SLC1A5 inhibits autophagy-mediated CD276 degradation to promote esophageal cancer progressionCancer Biology & TherapyDecember 31, 2026Chunyan Wang, Hongyan Zhang, Chaonan Guan, Yuying Li, Shengli Yang, Lan Huangovernight at 4 °C with primary antibodies: anti-CD276 (#740074T, Thermo Fisher Scientific), anti-SLC1A5 (#8057, Cell Signalling Technology) or anti-LAMP1(#9091S, Cell Signalling Technology) or isotype controls (rabbit

(2) ASCT2 palmitoylation regulated by JNK1-ZDHHC14 axis orchestrates glutamine metabolism and NSCLC progressionCell DiscoveryFebruary 24, 2026Xingyu Chen, Zihao Ke, Shihui Wei, Jiajin Chen, Ke Zhu, Jiaqi Xu, Yun Zhao, Mengyuan Cen, Yan Jin, Zhilei Pan, et al.(#SQ202L, EpiZyme). The antibodies used in this study are as follows: anti-ASCT2 (#A23156, ABclonal; #8057S, Cell Signaling Technology; #ab237704, Abcam), anti-Flag (#AE063, ABclonal), anti-MYC (#AE070, ABclonal),

(3) RHOJ derived peptide promotes chemosensitivity by inhibiting glutamine metabolism in gastric cancerJournal of Translational MedicineJanuary 28, 2026Jian Li, Huanqing Li, Fangzhou Ye, Jiayi Wang, Fan Li, Songhua Bei, Xiaohong Zhang, Jun Jiang, Li Fengused for WB were as follows: anti-GLUL (1:1,000; Cat# 80636S), anti-ASCT2 or SLC1A5 (1:1,000; Cat# 8057S), anti-bax (1,000; Cat# 5023S), anti-bcl2 (1:1,000; Cat# 3498S), and anti-C-capase3 (1:1,000; Cat# 9661T)

(4) Variations in blood levels of the anti-fusogenic endogenous retroviral protein suppressyn are associated with fetal growth outcome in preeclampsiaScientific ReportsNovember 3, 2025Jun Sugimoto, Danny J. Schust, Takeshi Nagamatsu, Eriko Yano, Makiko Sugimoto, Tomomi Yamazaki, Yoshiki Kudomonoclonal anti-suppressyn antibody (1/2000 dilution)34 or polyclonal anti-ASCT2 antibody (1/ 3000 dilution, 8057: Cell signaling technology, MA, USA), monoclonal anti-β-actin (1/10000 dilution, A5441: Sigma-Aldrich,

(5) SF3A3 Drives Tumorigenesis in Endometrial Cancer by Enhancing c‐FOS Expression and Represents a Potential Therapeutic TargetAdvanced ScienceSeptember 1, 2025Wei Yu, Yi Gu, Hengrui Zhang, Wunan Huang, Lei Sun, Liangjian Ma, Xiaolei Liang, Lexin Liu, Guiping Chen, Weihua Wang, et al.sliced into 8 µm sections and stained with primary antibodies against SF3A3 (Cell Signaling Technology, #8057S) or Ki67 (Abclonal #A2094) followed by anti‐rabbit IgG secondary antibodies (Cell Signaling Technology,

(6) Genetic Diversity in the Suppressyn Gene Sequence: From Polymorphisms to Loss-of-Function MutationsBiomoleculesJuly 21, 2025Jun Sugimoto, Danny J. Schust, Takeshi Nagamatsu, Yoshihiro Jinno, Yoshiki KudoLouis, MO, USA), polyclonal anti-DDDDK-tag (PM020; MBL, Tokyo, Japan), polyclonal anti-ASCT2 antibody (8057: Cell signaling technology, Danvers, MA, USA), and monoclonal anti-β-actin (A5441: Sigma-Aldrich, St.

(7) Large-scale experimental assessment of variant effects on the structure and function of the citrate transporter SLC13A5Sci AdvJune 27, 2025Wen-An Wang, Evandro Ferrada, Christoph Klimek, Tanja Osthushenrich, Aidan MacNamara, Tabea Wiedmer, Giulio Superti-Furgaanti-HA (rat) (Roche, 47877600), 1:1000; SLC1A5/ASCT2 (D7C12) (rabbit) (Cell Signaling Technology, 8057), 1:500; and calreticulin (rabbit) (Abcam, ab92516), 1:250. Secondary antibody staining was performed

(8) PLK1-mediated phosphorylation of PHGDH reprograms serine metabolism in advanced prostate cancerbioRxivMay 27, 2025Xiongjian Rao, Derek B Allison, Robert M Flight, Penghui Lin, Daheng He, Zhiguo Li, Yanquan Zhang, Ruixin Wang, Chaohao Li, Jianlin Wang, et al.PSPH(Peoteintech,Cat#14513–1-AP), ASCT1 (CST,Cat#8442S and Proteintech,Cat#13067–2-AP), ASCT2 (CST,Cat#8057S and Proteintech,Cat#20350–1-AP), ACC(CST,Cat#3676S), p-ACC(CST,Cat#11818S), FASN (CST, Cat#3189S), SPHK1

(9) RTN4IP1 Contributes to ESCC via Regulation of Amino Acid TransportersAdvanced ScienceJanuary 5, 2025Huifang Wei, Dengyun Zhao, Yafei Zhi, Qiong Wu, Jing Ma, Jialuo Xu, Tingting Liu, Jing Zhang, Penglei Wang, Yamei Hu, et al.1:1000), anti‐IRP2 (Cell signaling technology, #37135, 1:1000), anti‐SLC1A5 (Cell signaling technology, #8057, 1:1000), anti‐SLC3A2 (Santa Cruz Biotechnology, sc‐376815, 1:100), anti‐SLC7A5 (Cell signaling technology,

(10) ATF4 promotes glutaminolysis and glycolysis in colorectal cancer by transcriptionally inducing SLC1A5Acta Biochimica et Biophysica SinicaDecember 18, 2024Zengli Zhou, Shufang Ye, Jingyu Chen, Fei Dai, Luyi Chen, Ran Ye, Jianmei Zhang, Gefei Chen, Yanjiao Wang, Yangyang Liuimmunoreacted with primary antibodies against ATF4 (11815; Cell Signaling Technology, Danvers, USA) and SLC1A5 (8057S; Cell Signaling Technology), followed by incubation with horseradish peroxidase-labelled secondary antibodies

(11) Engineered packaging cell line for the enhanced production of baboon-enveloped retroviral vectorsMolecular Therapy - Nucleic AcidsDecember 10, 2024Denise Klatt, Lucia Sereni, Boya Liu, Pietro Genovese, Axel Schambach, Els Verhoeyen, David A. Williams, Christian BrendelTBST buffer, ASCT2 or B2M were detected using a monoclonal rabbit-anti-human ASCT2 antibody (CST, no. 8057, 1:1,000) or a monoclonal rabbit-anti-human B2M antibody (Abcam, no. 75853, 1:4,000) as primary antibody

(12) Prognostic significance of LAT1 expression in pleural mesotheliomaHeliyonSeptember 15, 2024Ryo Taguchi, Kyoichi Kaira, Yu Miura, Tetsuya Umesaki, Atsuto Mouri, Hisao Imai, Hiroshi Kagamu, Masanori Yasuda, Yoshikatsu Kanai, Hiroyuki Nitanda, et al.Glostrup, Denmark; M7240), ASCT2 rabbit monoclonal antibody (D7C12, 1:100 Cell Signaling Technology, Inc., #8057), mouse monoclonal CD8 (DAKO, clone C8/144B 1/150 high pH retrieval), and mouse monoclonal FOXP3 (Abcam,

(13) E-Cadherin Induces Serine Synthesis to Support Progression and Metastasis of Breast CancerCancer ResearchSeptember 4, 2024Geonhui Lee, Claudia Wong, Anna Cho, Junior J. West, Ashleigh J. Crawford, Gabriella C. Russo, Bishwa Ranjan Si, Jungwoo Kim, Lauren Hoffner, Cholsoon Jang, et al.(#5605S), PDH (#3205S), phospho-PDH (#31866), Smad2/3 (#8685S), HIF-1α (#14179S), HIF-2α (#7096S), ASCT2 (#8057) and E-cadherin (#3195S, #14472S) antibodies were obtained from Cell Signaling Technology for immunoblot

(14) Distinct roles and molecular mechanisms of nicotine and benzo(a)pyrene in ferroptosis of lung adenocarcinoma and lung squamous cell carcinomaTobacco Induced DiseasesJune 29, 2024Min G. Wen, Hui X. Zheng, Ying Z. Zhao, Pu Xiathe first antibody overnight at 4°C. Primary antibodies were FDFT1 (sc-271602, Santa Cruz), SLC1A5 (8057, Cell Signaling Technology, Shanghai, China), GPX4 (sc-166570, Santa Cruz) and GAPDH (sc-74512, Santa Cruz

(15) Glutamine promotes human CD8(+) T cells and counteracts imiquimod-induced T cell hyporesponsivenessiScienceMay 17, 2024Bopp L, Martinez ML, Schumacher C, Seitz R, Arana MH, Klapproth H, Lukas D, Oh JH, Neumayer D, Lackmann JW, et al.RRID:AB_10714077" Unconjugated monoclonal rabbit anti-ASCT2 antibody (clone D7C12),Cell Signaling Technologies,"Catalog No. 8057, RRID:AB_10891440" Unconjugated polycloncal rabbit anti-Phospho-S6 Ribosomal Protein (Ser235/236) antibody,Cell Signaling Technologies

(16) Activation of polyamine catabolism promotes glutamine metabolism and creates a targetable vulnerability in lung cancerProceedings of the National Academy of SciencesMarch 26, 2024Xinlu Han, Deyu Wang, Liao Yang, Ning Wang, Jianliang Shen, Jinghan Wang, Lei Zhang, Li Chen, Shenglan Gao, Wei-Xing Zong, et al.(Millipore), and detected with antibodies as follows: Primary antibodies. ASCT2 (Cell Signaling Technology, 8057S, 1:2,000), GLS (KGA/GAC, Proteintech, 12855-1-AP, 1:1,000), SLC7A11 (Proteintech, 12855-1-AP, 1:1,000),

(17) Basal MET phosphorylation is an indicator of hepatocyte dysregulation in liver diseaseMolecular Systems BiologyJanuary 12, 2024Sebastian Burbano de Lara, Svenja Kemmer, Ina Biermayer, Svenja Feiler, Artyom Vlasov, Lorenza A D’Alessandro, Barbara Helm, Christina Mölders, Yannik Dieter, Ahmed Ghallab, et al.hepatocyte experiments pMET Tyr1234/1235,Rabbit,1:2000,Cell Signaling,#3077 MET,Mouse,1:500,Santa Cruz,#8057 pAKT Thr308,Rabbit,1:2000,Cell Signaling,#4056 pAKT Ser473,Rabbit,1:2000,Cell Signaling,#4058 AKT,Rabbit,1:2000,Cell Signaling

(18) Mutant p53 sustains serine-glycine synthesis and essential amino acids intake promoting breast cancer growthNature CommunicationsOctober 25, 2023Camilla Tombari, Alessandro Zannini, Rebecca Bertolio, Silvia Pedretti, Matteo Audano, Luca Triboli, Valeria Cancila, Davide Vacca, Manuel Caputo, Sara Donzelli, et al.anti-CD98hc/SLC3A2 (1:2000, Sigma-Aldrich, HPA017980); anti-ASCT2/SLC1A5 (1:2000, Cell Signaling Technology, #8057); anti-PSAT1 (1:2000, Proteintech, 10501-1-AP); anti-eIF2α D7D3 (1:5000, Cell Signaling Technology,

(19) A Carbonic Anhydrase IX/SLC1A5 Axis Regulates Glutamine Metabolism Dependent Ferroptosis in Hypoxic Tumor CellsMolecular Cancer TherapeuticsOctober 2, 2023Geetha Venkateswaran, Paul C. McDonald, Shawn C. Chafe, Wells S. Brown, Zachary J. Gerbec, Shannon J. Awrey, Seth J. Parker, Shoukat Dedhar(R and D Systems, AF2188, RRID:AB_416562) – 1:1,000; rabbit anti-human ASCT2 (Cell Signaling Technology, 8057, RRID:AB_10891440) – 1:500; mouse anti-vinculin (Millipore, MAB3574, RRID:AB_2304338) – 1:1,000; rabbit

(20) Clinicopathological impact of VEGFR2 and VEGF‐C in patients with EGFR ‐major mutant NSCLC receiving osimertinibThoracic CancerOctober 1, 2023Kyoichi Kaira, Hisao Imai, Atsuto Mouri, Kosuke Hashimoto, Yu Miura, Ayako Shiono, Ou Yamaguchi, Kunihiko Kobayashi, Tomonori Kawasaki, Masanori Yasuda, et al.1:100 Dako; M7240) and ASCT2 rabbit monoclonal antibody (D7C12, 1:100 Cell Signaling Technology, Inc #8057) were quantified based on previous procedures. 12 High and low expression levels were defined as the

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ASCT2 (D7C12) Rabbit mAb

ASCT2 (D7C12) Rabbit mAb from Cell Signaling Technology

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ASCT2 (D7C12) Rabbit mAb from Cell Signaling Technology

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