Fig 2: Effects of treatment on iron metabolism in AS mice. The model group exhibited significant increases in serum iron (A) liver iron (B) aortic iron (C) HEP (D) FPN (E) expression compared to the control group. The 4 treatment groups showed significant reductions compared to the model group. Data are shown as means ± SEM. Compared with control group, *P < 0. 05, **P < 0. 01, ***P < 0. 001; compared with model group, #P < 0. 05, ##P < 0. 01, ###P < 0. 001. n = 7.

Fig 3: Effects of treatment on iron metabolism and ferroptosis related protein expression levels in AS mice. The model group exhibited significant increases in HEP, FPN, P53, PTGS2, and NOX1 protein levels, while GPX4, SLC7A11, and FTH1 levels were notably decreased compared to the control group. All 4 treatment groups significantly reversed these abnormal protein expressions (A). Quantitative results are shown in (B–I). Data are shown as means ± SEM. Compared with control group, *P < 0. 05, **P < 0. 01, ***P < 0. 001; compared with model group, #P < 0. 05, ##P < 0. 01, ###P < 0. 001. n = 3.