Fig 1: STOX1‐A promotes proliferation and growth of HCC cells by transcriptionally upregulating CCNB1. (A–D) The effect of STOX1‐A overexpression or downexpression on cell cycle progression of HCC cells, including G0/G1 phases (B), S phase (C) and G2/M phases (D), using flow cytometry. (E, F) Western blot analysis of the effect of STOX1‐A overexpression or downexpression on the expression of G1/S‐related regulatory proteins, including CDK2, CDK4, CDK6, cyclin D1 and cyclin E1, and G2/M‐related regulatory protein, including cyclin A1, cyclin B1 and CDK1. (G) Real‐time PCR analysis of the effect of STOX1‐A overexpression or downexpression on cyclin B1 expression. (H) Analysis of the effect of STOX1‐A overexpression or downexpression on the luciferase activity of cyclin B1 promoter using luciferase activity assay. * indicates p < 0.05.