Fig 2: POU2F2+B cells were associated with better immune infiltration in NSCLC. (a,b) mIHC staining and spatial distance calculations for CD20, POU2F2, CD8, and PD-1 in LUAD (a) and LUSC (b). (c,d) mIHC staining and spatial distance calculations for CD20, POU2F2, CD68, and CD206 in LUAD (c) and LUSC (d). (e,f) The relationship between POU2F2+ B cell score and immune cell infiltration in TCGA-LUAD (e) and TCGA-LUSC (f). ns not significant, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Fig 3: POU2F2 is highly expressed in tumor infiltration B cell. (a,b) UMAP plots showing different cell types and the expression of POU2F2, CD2, and CST7 in LUAD scRNA-seq datasets (GSE139555, GSE127465). (c,d) mIHC images showing the colocalization of POU2F2 and CD20 in LUAD (c) and LUSC (d). Scale bar: 100 μm, 20 μm. (e) Violin plot showing B cell groups based on POU2F2 expression levels. (f) GO enrichment analysis results for B cells with differential POU2F2 expression. g) KEGG enrichment analysis results for B cells with differential POU2F2 expression.