Fig 1: Wnt5a-mediated inhibition of organoids growth requires NOTUM. (A) Organoids were treated with DMSO or Dox for 7days. RT–qPCR analysis of the expression of NOTUM and ATOH8. The data are presented as mean ± SEM (n = 3). (B) Protein association network for NOTUM using the STRING database. (C) Organoids were treated with DMSO or Dox for 7days. Western blot of NOTUM using anti-NOTUM. (D-E) Organoids were RT–qPCR (D) and Western blot (E) analysis of NOTUM expression in organoids treated with DMSO or Dox in the presence or absence of SB431542 for 7 days. The data are presented as mean ± SEM (n = 3). (F-G) Organoids were treated with or without TGF-β (10 ng/mL) in the presence or absence of SB431542 for 7 days. Expression of NOTUM mRNA (F) and NOTUM levels were blotted with anti-NOTUM (G). The data are presented as mean ± SEM (n = 3). (H) Representative images of organoids treated with DMSO or DOX in the presence or absence of ABC99 (500 nM) for 7 days. Scale bars: 100 μm. Quantification of organoid sizes in (H). (I) Organoids were treated with or without Dox in the presence or absence of ABC99 for 7 days. OLFM4 levels were blotted with anti-OLFM4