Fig 1: Western blot analysis of NF-κB and proteins associated with invasion. Experiments were repeated three times. (A) western blot images and (B) densitometric analysis of proteins; *P<0.05 vs. NC, **P<0.01 vs. NC. TOPO II, topoisomerase II; NF-κB, nuclear factor κB; MMP, metalloproteinase; IκB, inhibitor of κB; R-CAD, retinal cadherin; N-CAD, neural cadherin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NC, negative control; Bay117082, inhibitor of the NF-κB signaling pathway. Data for each protein were analyzed with one-way ANOVA and Dunnett's post hoc test.
Fig 2: Effect of CDH4 on α-synuclein aggregation. A) The OLN-AS7 oligodendrocyte MSA cell model was transfected with CDH4 cDNA or empty-vector control (Con) and α-synuclein expression measured by qPCR. B) Control cells and (C) CDH4-transfected cells were immunofluorescent-stained and examined under a confocal microscope. D) A comparion of α-synuclein signal intensity of the two groups. Data represent mean and SE as error bars; *p < 0.05.
Fig 3: CDH4 mRNA expression in control and MSA brain. A) Relative mRNA expression of CDH4 in control (Con), MSA-P (M-P), and MSA-C (M-C) in the putamen, motor cortex white matter, cerebellum, and superior occipital cortex. Data represent mean and SE as error bars; * p < 0.05. B) A comparison of mRNA expression in the putamen (P), motor cortex white matter (M), cerebellum (C), and superior occipital cortex (O) within each cohort. The box plots depict mean with maximum and minimum values; *p < 0.05.
Fig 4: CDH4 protein expression in control and MSA brain. A) CDH4 protein expression in control (Con), MSA-P (M-P), and MSA-C (M-C) in the motor cortex white matter and superior occipital cortex as measured by western blotting. The CDH4 bands were normalized to β-actin. Data represent mean and SE as error bars; *p < 0.05. B) Correlation of CDH4 and α-synuclein protein levels in the motor cortex white matter.
Fig 5: Effect of CDH4 on α-synuclein levels. MO3.13 oligodendrocyte cells were transfected with CDH4 cDNA or empty-vector control (Con) and α-synuclein expression measured by qPCR (A) and western blotting (B). Optical density of α-synuclein monomeric (M) and trimeric (T) bands were measured and normalized to GAPDH. Data represent mean and SE as error bars; *p < 0.05.
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