Fig 2: Lnc-TLN2-4:1 expression is associated with overall survival rates of GC patients. (a) ROC curve shows that the expression of lnc-TLN2 has an AUC of 0.7071 in distinguishing GC tissue from nontumor tissue, with the sensitivity of 65.31% and the specificity of 81.25% and the cutoff value of 1.246. (b) ROC curve shows that the expression of lnc-TLN2 has an AUC of 0.733 in distinguishing GC tissue from nontumor tissue, with the sensitivity of 70.97% and the specificity of 72.22% and the cutoff value of 1.016. (c) Overall survival analysis shows the survival rates of GC patients with low or high expression of lnc-TLN2-4:1, which is defined by the cutoff value of 1.246. (d) Overall survival analysis shows the survival rates of GC patients with low or high expression of lnc-TLN2-4:1, which is defined by the cutoff value of 1.016.

Fig 3: Lnc-TLN2-4:1 represses GC cell migration and invasion in vitro. (a) Scatter plots show the expression of lnc-TN2-4:1 in 49 nontumor tissues and GC cell lines, detected by qRT-PCR, and ß-actin serves as the internal control. (b and c) Bars show the expression of lnc-TLN2-4:1 in BGC823 and SGC7901 cells which were transfected with lnc-TLN2-4:1-overexpressing vectors, detected by qRT-PCR, and ß-actin serves as the internal control. (d and e) Wound healing experiments show the abilities of BGC823 and SGC7901 cells which were transfected with lnc-TLN2-4:1-overexpressing vectors to migrate. Bars show the statistics based on three independent experiments. Area indicates the area without cells in the images, calculated by Image J. (f and g) Transwell experiments show the ability of BGC823 and SGC7901 cells which were transfected with lnc-TLN2-4:1-overexpressing vectors to invasive. Bars show the statistics based on three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Fig 4: Lnc-TLN2-4:1 is located in the GC cell cytoplasm, and its expression is positively correlated with TLN2 expression in GC tissues. (a) Fluorescence in situ hybridization (FISH) shows the expression and location of lnc-TLN2-4:1 in BGC823 cells. DAPI indicates cell nucleus. GFP indicates the expression status of the vectors (negative control and lnc-TLN2-4:1-overexpressing vectors). Red fluorescence indicates the expression of lnc-TLN2-4:1. (b and c) Bars show the relative expression % of lnc-TLN2-4:1 in the cytoplasm and nucleus of BGC823 cells which were transfected with the control or lnc-TLN2-4:1-overexpressing vectors, detected by qRT-PCR, and ß-actin serves as the internal control in the cytoplasm and U6 serves as the internal control in the nucleus. (d) Bars show the expression of TLN2 in BGC823 and SGC7901 cells which were transfected with control or lnc-TLN2-4:1-overexpressing vectors, detected by qRT-PCR, and ß-actin serves as the internal control. (e) Western blotting shows the protein expression of TLN2 in BGC823 and SGC7901 cells which were transfected with the control or lnc-TLN2-4:1-overexpressing vectors, and GAPDH serves as the internal control. Bars show the statistics based three independent experiments. (f) The correlation between the expression of TLN2 mRNA and lnc-TLN2-4:1 in 49 pairs of GC tissues, detected by qRT-PCR, and ß-actin serves as the internal control. *P < 0.05, ***P < 0.001, and ****P < 0.0001.