Fig 1: IQGAP1 preferentially binds to CLIP170 over DIAPH1.a Preassembled GST-IQGAP1-DBR and His6-DIAPH1-NT complexes were incubated with MBP-CLIP170-NT. In parallel preassembled GST-IQGAP1-DBR and MBP-CLIP170-NT complexes were incubated with His6-DIAPH1-NT. After reisolating GST-IQGAP1-DBR, the amount of co-purifying MBP-CLIP170-NT or His6-DIAPH1-NT was determined. NT = N-terminus, DBR = Diaphanous Binding Region. b Schematic representing inferred IQGAP1 interaction dynamics.
Fig 2: The CLIP170-IQGAP1 interaction is dependent upon anaphase astral microtubules.a Time-lapse images of GFP-CLIP170 during cell division. b Proximity ligation assays (PLA) mapping the proximity DIAPH1 to IQGAP1 (top), IQGAP1 to CLIP170 (middle) and CLIP170 to DIAPH1 (bottom) during mitotic progression. c Quantitation of PLA foci in metaphase and anaphase *p < 0.0001 using a nonparametric two-tailed Mann-Whitney t-tests for three experimental repeats, n = 50 cells examined over three independent experiments. d Proximity ligation assays (PLA) mapping the proximity DIAPH1 to IQGAP1 and IQGAP1 to CLIP170 in Centrinone B-treated cells. e Quantitation of PLA foci in d. At cell poles lacking astral microtubules, DIAPH1 and IQGAP1 are in close proximity but IQGAP1 and CLIP170 are not. *p = 0.0006, **p < 0.0001 using nonparametric two-tailed Mann-Whitney t-tests for three experimental repeats, n = 50 cells examined over three independent experiments. Scale bars are 10 µm.
Supplier Page from Abcam for Anti-CLIP170 antibody