Fig 1: PON2 and RAB4A promoted LRIG1-mediated down-regulation of PDGFRA expression. In the triple co-transfection system, cells were co-transfected with LRIG1, PDGFRA, and individual shRNA vectors targeting the LRIG1 interactors RAB4A and PON2 as described in the legend to Fig. 1. A, representative Western blots showing RAB4A, PON2, and PDGFRA expression in HEK293 cells triple–co-transfected with LRIG1, PDGFRA, and different shRNAs targeting RAB4A (left) or PON2 (right). Vertical black lines, non-adjacent lanes on the same blot. B, quantification of RAB4A (left) and PON2 (right) protein levels after triple co-transfections with LRIG1, PDGFRA, and the indicated shRNAs. The results from four independent experiments similar to A are shown. C, quantification of PDGFRA protein levels from four biological replicates as described in B. Darker and lighter gray bars represent the upper and lower PDGFRA bands, respectively. Statistical analyses were performed as described in the legend to Fig. 1. Error bars, S.D.