Western blot was performed using Anti-NTHL1 Polyclonal Antibody (Product # PA3-16563) and a 34 kDa band corresponding to Endonuclease III-like protein 1 was observed across the cell lines and tissues tested and increased upon serum release for 24 Hours followed by serum starvation for 72 Hours in HaCa T. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2(Lane 2), Reh (Lane 3), A-431 (Lane 4), HaCa T serum starvated for 72 Hours (Lane 5), HaCa T serum release for 24 Hours followed by serum starvation for 72 Hours (Lane 6) and Mouse Brain (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).