Fas (4C3) Mouse mAb 8023S from Cell Signaling Technology

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Experimental and Therapeutic Medicine
July 23 2021

Fig 1: Overexpression of NF-?B reversed the Fas knockdown-mediated promotion of apoptosis and inhibition of cell migration. (A) Protein levels of NF-?B, Bcl-2 and Bax in trophoblast cells transfected with OE-Fas, OE-NC, shRNA-Fas and KD-NC examined using western blot analysis. The protein levels of (B) NF-?B, (C) Bcl-2 and (D) Bax were quantified by densitometry and normalized to the expression of ß-actin. (E) Measurement of the mRNA level of NF-?B p65 in the trophoblast cells confirmed the transfection efficiency of the NF-?B p65 overexpression vector. (F) Trophoblast cell apoptosis in the sh-Fas + p65 and sh-Fas + OE-NC groups was evaluated using flow cytometry and the percentage of apoptotic cells in each group is shown. (G) The migration ability of trophoblast cells in the sh-Fas + p65 and sh-Fas + OE-NC groups was examined using a Transwell assay (magnification, x200). Quantification of the migration rate in three separate experiments is shown. (H) Representative western blots showing the protein levels of NF-?B, Bcl-2 and Bax in the sh-Fas + p65 and sh-Fas + OE-NC groups of trophoblast cells. Protein levels of (I) NF-?B, (J) Bcl-2 and (K) Bax were quantified by densitometry and normalized to the expression of ß-actin. Results are expressed as the mean ± SD (n=3). **P<0.01 and ***P<0.001. OE, overexpression; NC, negative control; KD, knockdown; sh, short hairpin; NF-?B, nuclear factor ?B.
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Scientific Reports
December 01 2022

Fig 2: TY upregulated the expression of Fas that functions as a caspase-8-dependent apoptosis inducer. (A) Immunocytochemistry for Fas in TY-treated cells. The expression levels of Fas in the cytoplasm increased dose-dependently (4, 5, and 6 µg/mL). Nuclear Hoechst 33348 staining was also performed. (B) Fas-activating anti-Fas antibody (CH-11) synergistically induced apoptotic cell death by co-treatment with TY, which was estimated by the viable cell rate and Hoechst 33342 staining. (C) TRAIL-blocking anti-TRAIL antibody (2E5) partly canceled apoptotic cell death induced by TY through suppressing the activation of caspase-8, which was estimated by the viable cell rate and Western blot analysis of caspase-8.
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Clinical and Translational Medicine
June 06 2021

Fig 3: Therapeutic impact of atglistatin on iWAT post-burn injury: Twelve-week-old C57BL/6 mice were treated with 30%TBSA injury and treated with Atglistatin (2 mmol/kg i.p.) starting at day 1 and monitored daily for 7 days. (A) Atglistatin treatment study plan. (B) Tri-acylglycerol (TAG) content in serum samples. (C) Free fatty acid levels in serum samples. (D) TAG content in liver samples normalized to tissue weight. (E) Adipocyte size of adipocytes in iWAT. (F) Percentage of multilocular adipocytes normalized to total adipocytes. (G) Hematoxylin and eosin (H&E) staining and immunohistochemistry for UCP1 expression in WAT samples. (H and I) Basal, and state 3 respiration parameters were measured in isolated mitochondria using Seahorse assays. Quantitative PCR analysis of (J) browning markers (Ucp1, Pgc1a, Cd137, and Tmem26), (K) lipid synthesis markers (Fas, Scd1, and Pparg), and (L) lipolysis markers (Atgl and Hsl), lipid oxidation (Cpt1a), lipid uptake (Cd36), and hepatokine (Fgf21) markers in WAT. The results displayed are the average and SEM analyzed in the specified number of mice samples. Statistical significance was assessed using one-way ANOVA tests as appropriate
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Clinical and Translational Medicine
June 06 2021

Fig 4: Adipose-specific ATGL deletion rescues against fatty liver development post-burn injury: Twelve-week-old ATGL floxed and knockout mice were treated with 30%TBSA injury and monitored daily for 7 days. (A) Oil Red O staining (zoom in and zoom out) for visualization of fat droplets in liver sections. (B) Tri-acylglycerol (TAG) content in liver samples normalized to tissue weight. (C) Aspartate aminotransferase (AST) levels in serum samples. (D) Alanine aminotransferase (ALT) levels in serum samples. (E) Western blot analysis for the expression of targeted proteins (p-HSL ser 563, HSL, ATGL, and actin) in the liver. (F) Western blot analysis and quantification of the expression of targeted proteins (Fas, Scd1, and gapdh) in the liver. (G) Quantitative PCR analysis of multiple genes in the liver. The results displayed are the average and SEM analyzed in the specified number of mice samples. Statistical significance was assessed using two-way ANOVA as appropriate
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Scientific reports
October 31 2017

Fig 5: Confocal immunofluorescence analysis of iCCA cells. (A) c-FLIP and Fas double labeling of iCCA cells cultured alone and after 24 h of co-culture with PBMCs. Scale bar = 10 µm. (B) Pseudocolor analysis of c-FLIP immunolabeling in both iCCA cells. Histograms reported on the right side of pseudocolor images represent the mean ± SD of the ratio of nucleus/cytoplasm expression of c-FLIP in both cancer cell types cultured alone, in comparison with cancer cells co-cultured with PBMCs (***P < 0.001). (C) Specificity control of c-FLIP antibody. Sample was counterstained with DAPI and anti-Fas Ab. Fluorescence images were superimposed to DIC image. (D) Triple immunofluorescence of mixed-iCCA cells by: DAPI; anti-c-FLIP Ab detected by Alexa488 conjugated secondary Ab; anti-Fas Ab detected by alexa546 conjugated secondary Ab. Spectral confocal analysis of signals from DAPI, alexa488 and alexa546 fluorochromes was shown in the graphs on the right side. Theoretical emission spectra of fluorochromes was represented in the respective color (DAPI in blue; alexa488 in green; alexa546 in red) whereas real emission spectrum was represented in white. Circles in the image indicate areas of analysis.

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Cell Signaling Technology for
Fas (4C3) Mouse mAb

Cell Signaling Technology's Fas (4C3) Mouse mAb is a Mouse monoclonal antibody. The Fas (4C3) Mouse mAb Antibody was generated using Fas as the antigen. It reacts with Homo Sapiens, and Human. This antibody has been shown to work in applications such as: Precipitation, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blot.

Description

Fas (4C3) Mouse mAb recognizes endogenous levels of total Fas protein

Product Specs
ItemFas (4C3) Mouse mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number8023S
Quantity100 µl
ApplicationsW, IP, IF-IC, FC-FP
ReactivityH
TargetFas
HostMouse
NCBI Gene AliasesALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6
ClonalityMonoclonal
NCBI Full Gene NameFas cell surface death receptor
Research AreaApoptosis
IsotypeIgG1
Clone4C3
Molecular Weight40-50

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Cell Signaling Technology

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Phone: 978-867-2388
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Website: http://www.cellsignal.com/

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(1) Leaf extract of Osbeckia octandra induces apoptosis in oral squamous cell carcinoma cellsBMC Complementary Medicine and TherapiesDecember 1, 2022Jue Young Kim, Jin Kim, B. M. Ratnayake Bandara, Wanninayake M. Tilakaratne, Dokyeong KimMA, USA), and Cleaved Caspase-3 (Asp175, 1:1000, Cell Signaling Technology, Danvers, MA, USA), Fas (#8023 s, 1:1000, Cell Signaling Technology, Danvers, MA, USA), Caspase-8 (#9746 s, 1:1000, Cell Signaling Technology

(2) Understanding of cell death induced by the constituents of Taxus yunnanensis woodScientific ReportsDecember 1, 2022Yukihiro Akao, Riyako Terazawa, Nobuhiko Sugito, Kazuki Heishima, Kohei Morikawa, Yuko Ito, Ryoko Narui, Reo Hamaguchi, Takahiro Nobukawausing Image J (version 2.0.0-rc-69/1.52p, NIH). Primary antibodies used were as follows: anti-FAS (#8023), DR5 (#8074), TRAIL (#3219), cyclin D1 (#55506), cleaved caspase-8 (#9496), PARP (#9542), FADD (#2782),

(3) GM-CSF disruption in CART cells modulates T cell activation and enhances CART cell anti-tumor activityLeukemiaJune 1, 2022Michelle J. Cox, Claudia Manriquez Manriquez Roman, Erin E. Tapper, Elizabeth L. Siegler, Dale Chappell, Cameron Durrant, Omar Ahmed, Sutapa Sinha, Raphael Mwangi, Nancy S. Scott, et al.BID Antibody (human specific) (2002S, Cell Signaling, Danvers, MA, USA) (dilution 1:1000), Fas (4C3) Mouse mAb (8023S, Cell Signaling, Danvers, MA, USA), and β-Actin (D6A8) Rabbit mAb (8457T, Cell Signaling, Danvers, MA,

(4) Cotargeting of miR-126-3p and miR-221-3p inhibits PIK3R2 and PTEN, reducing lung cancer growth and metastasis by blocking AKT and CXCR4 signallingMolecular oncologyNovember 1, 2021Di Paolo D, Pontis F, Moro M, Centonze G, Bertolini G, Milione M, Mensah M, Segale M, Petraroia I, Borzi C, et al.anti‐TRAILR 2 (clone: D9S1R; #42533), rabbit anti‐FADD (Human Specific; #2782), mouse anti‐FAS (clone: 4C3; #8023), rabbit anti‐p21( Waf1/Cip1; clone: 12D1 #2947), rabbit anticleaved caspase 3 (clone: Asp175; #9661)

(5) Fas regulates the apoptosis and migration of trophoblast cells by targeting NF‑κBExperimental and Therapeutic MedicineJuly 23, 2021Ruihong Lan, Yang Yang, Jie Song, Ling Wang, Humin Gongcontaining 0.1% Tween 20. The membranes were then probed using primary antibodies against Fas (cat. no. 8023), Bax (cat. no. 14796), NF-κB p65 (cat. no. 8242), Bcl-2 (cat. no. 15071) and b-actin (cat. no. 3700).

(6) Adipose‐specific ATGL ablation reduces burn injury‐induced metabolic derangements in miceClinical and Translational MedicineJune 6, 2021Supreet Kaur, Christopher Auger, Dalia Barayan, Priyal Shah, Anna Matveev, Carly M. Knuth, Thurl E. Harris, Marc G. Jeschkephosphor‐HSL‐serine 563 (1:1000, Cell Signaling #4139), UCP1 (1:1000, Abcam ab234430), Fas (1:2000, Cell Signaling #8023), SCD1 (1:1000, Cell Signaling #2794), actin (1:5000, Cell Signaling #3700), gapdh (1:2000, Cell Signaling

(7) Etanercept Protected Against Cigarette Smoke Extract-Induced Inflammation and Apoptosis of Human Pulmonary Artery Endothelial Cells via Regulating TNFR1International Journal of Chronic Obstructive Pulmonary DiseaseMay 11, 2021Hong Xue, Baosong Xie, Nengluan Xu, Hongru Li, Qianshun Chen, Weiping Xie, Hong Wanganti-Bcl-2 (B-cell lymphoma 2) antibody (mouse, 1:1000, #15071, CST), anti-Fas antibody (mouse, 1:1000, #8023, CST), anti-FasL (Fas ligand) antibody (rabbit, 1:1000, #68405, CST), anti-TNFR1 antibody (rabbit, 1:1000,

(8) Antitumor activity of tetrandrine citrate in human glioma U87 cells in vitro and in vivoOncology ReportsOctober 15, 2019Jingyu Sun, Yang Zhang, Yongzhan Zhen, Ju Cui, Gang Hu, Yajun Lincleaved (CL) caspase-3 (cat. no. 9661; 1:1,000), caspase-3 (cat. no. 9662; 1:1,000), Fas (cat. no. 8023; 1:1,000), Bcl-2 (cat. no. 15071; 1:1,000), Bax (cat. no. 2772; 1:1,000), p-p38 (cat. no. 9216; 1:1,000),

(9) Resveratrol Maintains Lipid Metabolism Homeostasis via One of the Mechanisms Associated with the Key Circadian Regulator Bmal1MoleculesAugust 12, 2019Jing Li, Liping Wei, Caicai Zhao, Junyi Li, Zhigang Liu, Min Zhang, Yutang Wang(123456), p-GSK-3β (ser9, 9336), Sirt1 (1F3, 8469), ACC (3662), p-ACC (Ser 79, 11818), and FAS (4C3, 8023) were purchased from Cell Signaling Technology Company (Shanghai, China). The p-IRS-1 (Tyr 612) antibody

(10) Phenylhexyl isothiocyanate suppresses cell proliferation and promotes apoptosis via repairing mutant P53 in human myeloid leukemia M2 cellsOncology LettersJuly 16, 2019Yong Zou, Yiqun Huang, Xudong Ma(cat. no. #2947), p53 (cat. no. #2527), Bcl‑2 (cat. no. #4223), Bax (cat. no. #5023), Fas (cat. no. #8023), α‑tubulin (cat. no. #3873) and β‑actin (cat. no. #3700) were purchased from Cell Signaling Technology,

(11) The knocking down of the oncoprotein Golgi phosphoprotein 3 in T98G cells of glioblastoma multiforme disrupts cell migration by affecting focal adhesion dynamics in a focal adhesion kinase-dependent mannerPLOS ONEFebruary 19, 2019Cecilia Arriagada, Charlotte Luchsinger, Alexis E. González, Tomás Schwenke, Gloria Arriagada, Hugo Folch, Pamela Ehrenfeld, Patricia V. Burgos, Gonzalo A. Mardones

(12) Curcumin Inhibits Proliferation of Epstein-Barr Virus-Associated Human Nasopharyngeal Carcinoma Cells by Inhibiting EBV Nuclear Antigen 1 ExpressionBioMed research internationalJanuary 1, 2019Liu L, Yang J, Ji W, Wang C.sc-56, 196; Santa Cruz), antiGAPDH (Cat. #10494-1-AP; Proteintech, Wuhan, Hubei, China), anti-Fas (Cat. #8023; CST), and anti-FasL (Cat. #4273; CST), respectively, at 4°C overnight. Then, the membrane was washed

(13) Cholinergic Receptor Nicotinic Alpha 5 (CHRNA5) RNAi is associated with cell cycle inhibition, apoptosis, DNA damage response and drug sensitivity in breast cancerPLOS ONEDecember 13, 2018Sahika Cingir Koker, Ermira Jahja, Huma Shehwana, Ayse Gokce Keskus, Ozlen Konumouse, sc-47724, Santa Cruz Biotechnology); anti-Fas cell surface death receptor (FAS; Monoclonal Mouse, 8023, Cell Signaling); anti-Phospho-RB (Ser807/811) (pRB; Polyclonal rabbit, 9308, Cell Signaling, a kind

(14) Nrf2 negatively regulates STING indicating a link between antiviral sensing and metabolic reprogrammingNature CommunicationsDecember 1, 2018David Olagnier et al.

(15) Hepatitis B Virus Surface Antigen Enhances the Sensitivity of Hepatocytes to Fas-Mediated Apoptosis via Suppression of AKT PhosphorylationThe Journal of ImmunologyOctober 15, 2018Zhen-Tang Jing, Wei Liu, Shu-Xiang Wu, Yun He, Yan-Ting Lin, Wan-Nan Chen, Xin-Jian Lin, Xu Linof cells with chemicals and Abs The Journal of Immunology for 12 h at 4˚C using anti-Fas Ab (no. 8023; Cell Signaling Technology) and protein A/G–agarose (Santa Cruz Biotechnology). After IP, the beads were

(16) Integrative Analysis of Proteome and Ubiquitylome Reveals Unique Features of Lysosomal and Endocytic Pathways in Gefitinib-Resistant Non-Small Cell Lung Cancer CellsProteomicsAugust 1, 2018Li W, Wang H, Yang Y, Zhao T, Zhang Z, Tian Y, Shi Z, Peng X, Li F, Feng Y, et al.antibodies were used: HMGA2 (CST, #8179S), ALDH1A1 (CST, # 12035S), CHK2 (CST, # 3440S), FAS (CST, # 8023S), TGFBR1 (CST, # 3712S), Flag (Sigma‐Aldrich, #F3165), LC3B (CST, #3868), NUCB2 (Proteintech, #26712‐1‐AP),

(17) Golgi phosphoprotein 3 (GOLPH3) promotes hepatocellular carcinoma progression by activating mTOR signaling pathwayBMC cancerJune 18, 2018Liu H, Wang X, Feng B, Tang L, Li W, Zheng X, Liu Y, Peng Y, Zheng G, He Q.

(18) Corilagin induces the apoptosis of hepatocellular carcinoma cells through the mitochondrial apoptotic and death receptor pathwaysOncology ReportsApril 23, 2018Yuan Deng, Xudan Li, Xuan Li, Zhizhong Zheng, Wen Huang, Lianghua Chen, Qingxuan Tong, Yanlin MingmAb; cat. no. 9746), surviving (1:1,000; mouse mAb, cat. no. 2802), Fas (1:1,000; mouse mAb; cat. no. 8023) and FasL (1:1,000; rabbit mAb; cat. no. 4273) were purchased from Cell Signaling Technology (Danvers,

(19) Allicin induces apoptosis through activation of both intrinsic and extrinsic pathways in glioma cellsMolecular Medicine ReportsFebruary 2, 2018Chenlong Li, Hanguang Jing, Guangtao Ma, Peng Liangtemperature for 1 h and incubated separately with mouse anti‑human Fas (1:1,000 dilution, cat. no. 8023S; Cell Signaling Technology, Inc., Danvers, MA, USA), mouse anti‑human FasL (1:1,000 dilution, cat. no.

(20) Defects in the mitochondrial-tRNA modification enzymes MTO1 and GTPBP3 promote different metabolic reprogramming through a HIF-PPARγ-UCP2-AMPK axisScientific reportsJanuary 18, 2018Boutoual R, Meseguer S, Villarroya M, Martín-Hernández E, Errami M, Martín MA, Casado M, Armengod ME.

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Experimental and Therapeutic Medicine

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Clinical and Translational Medicine

Scientific reports

Fas (4C3) Mouse mAb from Cell Signaling Technology

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Cell Signaling Technology

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Fas (4C3) Mouse mAb from Cell Signaling Technology

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