SPARC (D10F10) Rabbit mAb 8725S from Cell Signaling Technology

Product Specs
ItemSPARC (D10F10) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number8725S
Quantity100 µl
ApplicationsW, IHC-P
ReactivityH, M
TargetSPARC
HostRabbit
NCBI Gene AliasesBM-40, OI17, ON, ONT
ClonalityMonoclonal
NCBI Full Gene Namesecreted protein acidic and cysteine rich
Research AreaDevelopmental Biology
IsotypeIgG
CloneD10F10
Molecular Weight42

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) High‐Calorie Diet During Pregnancy Leads to Muscular Fibrosis and Neuromuscular Damage in Offspring MiceJournal of Cachexia, Sarcopenia and MuscleOctober 1, 2025Jun Seok Son, Song Ah Chae, Yoon Ha Chun, Hongyang Wang, Zhihua Jiang, Min Duand PRDM16 (#PA5‐20872; RRID:AB_11154178) were purchased from Invitrogen (Rockford, IL, USA). SPARC (#8725; RRID:AB_10860770) and VDAC (#4661; RRID:AB_10557420) were also purchased from Cell Signaling Technology

(2) Deletion of EP3 prostaglandin receptor in murine macrophages aggravates diet-induced obesity by suppressing SPARCThe EMBO JournalJuly 23, 2025Wenlong Shang, Yinxiu Li, Lu Wang, Jiao Liu, Huiwen Ren, Qian Liu, Shumin Guo, Yuhong Wang, Yubo Ma, Tianyi You, et al.(#108512, 1:200; Biolegend); Dnmt1 (#ab188453, 1:100; Abcam); Dnmt3a (#188470, 1:1000; Abcam); SPARC (#8725S, 1:1000; Cell Signaling Technology); CD31(#ab28364, 1:50; Abcam) at 4 °C overnight. An isotype IgG antibody

(3) Pre-diagnostic Demographic, Lifestyle, and Health History Factors in Association with Secreted Protein Acidic and Rich in Cysteine (SPARC) Expression in Colorectal Cancer TissueBiomark InsightsMay 21, 2025Umaimah Zanif, Jaclyn Parks, Isabella Tai, Stephen Yip, Sindy Babinszky, Katy Milne, Peter Watson, Rachel A. Murphy, Parveen Bhattiantigen (SPARC D10F10; RRID:AB10860770) was obtained from Cell Signaling Technology (catalog number #8725) and used to stain the TMA slides on a Biocare Intellipath FLX autostainer. The slides were imaged using

(4) Elevated SPARC Disrupts the Intestinal Barrier Integrity in Crohn's Disease by Interacting with OTUD4 and Activating the MYD88/NF‐κB PathwayAdvanced ScienceJanuary 30, 2025Jiayu Wang, Yuxin He, Xingchao Zhu, Jinghan Zhu, Zilin Deng, Huan Zhang, Yanjun Chen, Guangbo Zhang, Tongguo Shi, Weichang Chenmeasured using a BCA protein assay kit (Beyotime). Primary antibodies specific to SPARC (1:1000, CST, #8725), ZO‐1 (1:10000, Proteintech, #21773‐1‐AP), Occludin(1:10000, Proteintech, #66378‐1‐Ig), OTUD4 (1:1000,

(5) MNK-driven eIF4E phosphorylation regulates the fibrogenic transformation of mesenchymal cells and chronic lung allograft dysfunctionThe Journal of clinical investigationAugust 15, 2024Walker NM, Ibuki Y, McLinden AP, Misumi K, Mitchell DC, Kleer GG, Lock AM, Vittal R, Sonenberg N, Garner AL, et al.total AKT (9272), phospho–p70 S6K1 (Thr389) (9234), total p70 S6K1 (2708), cyclin D1 (2978), SPARC (8725) and CD44 (3570) were purchased from Cell Signaling Technologies. Antibodies against phospho-eIF4E (Ser209)

(6) TGF-β1 upregulates secreted protein acidic and rich in cysteine expression in human granulosa-lutein cells: a potential mechanism for the pathogenesis of ovarian hyperstimulation syndromeCell communication and signaling : CCSMay 8, 2023Dang X, Fang L, Jia Q, Wu Z, Guo Y, Liu B, Cheng JC, Sun YP.(#MA5-13182). The α-tubulin (#sc-23948) antibody was purchased from Santa Cruz Biotechnology. The SPARC (#8725) antibody for immunohistochemistry was purchased from Cell Signaling Technology. The recombinant human

(7) Relationship between impaired BMP signalling and clinical risk factors at early-stage vascular injury in the preterm infantThoraxDecember 1, 2022Heydarian M, Oak P, Zhang X, Kamgari N, Kindt A, Koschlig M, Pritzke T, Gonzalez-Rodriguez E, Förster K, Morty RE, et al.Technology, #9511), SMAD4 (Santa Cruz Biotechnology, #7966), SPARC (D10F10, Cell Signalling Technologies #8725), VE-Cadherin (H-72, Santa Cruz Biotechnology #28644), VEGF-A (147, Santa Cruz Biotechnology #507),

(8) Loss of core-fucosylation of SPARC impairs collagen binding and contributes to COPDCellular and molecular life sciences : CMLSJune 7, 2022Wu TJ, Wang SH, Chen ES, Tsai HH, Chang YC, Tseng YH, Yu J.immunoprecipitations, the following antibodies were used: SPARC (Cell Signaling Technology; #5420, #8725), collagen type I (Sigma-Aldrich; AB765P; Santa Cruz, sc-59772), and a β-actin (Sigma-Aldrich; A5441).

(9) Tissue differences in the exosomal/small extracellular vesicle proteome and their potential as indicators of altered tissue metabolismCell reportsJanuary 18, 2022Garcia-Martin R, Brandao BB, Thomou T, Altindis E, Kahn CR.Santa Cruz); THBS1 (14778, Cell Signaling), ITGB1 (4706, Cell Signaling), EGFR (2232, Cell Signaling), SPARC (8725, Cell Signaling), IGFBP5 (AF578, R&D systems), CD9 (ab92726, Abcam), CD63 (ab68418, Abcam), CANX (ab22595,

(10) miR‑29a‑3p regulates the epithelial‑mesenchymal transition via the SPARC/ERK signaling pathway in human bronchial epithelial cellsInternational Journal of Molecular MedicineJuly 12, 2021Xiaobo Zhang, Jun Xie, Hongmei Sun, Qin Wei, Guangmin Nongincubated at 4°C overnight with primary antibodies against the following proteins: SPARC (cat. no. 8725; 1:1,000; Cell Signaling Technology, Inc.), E-cadherin (cat. no. 3195; 1:1,000; Cell Signaling Technology,

(11) The role of chromodomain helicase DNA binding protein 1 (CHD1) in promoting an invasive prostate cancer phenotypeTherapeutic advances in urologyJanuary 1, 2021Kareddula A, Medina DJ, Petrosky W, Dolfi S, Tereshchenko I, Walton K, Aviv H, Sadimin E, Tabakin AL, Singer EA, et al.(CST, 9102S), phosphorylated protein kinase B (pAKT) (CST, 4060S), total AKT (CST, 72), SPARC (CST, 8725), pMEK 1/2 (mitogen-extracellular signal-regulated kinase 1/2) (CST, 9154), total MEK 1/2 (CST, 9126),

(12) Pten regulates collagen fibrillogenesis by fibroblasts through SPARCPloS oneJanuary 1, 2021Jones CE, Sharick JT, Colbert SE, Shukla VC, Zent JM, Ostrowski MC, Ghadiali SN, Sizemore ST, Leight JL.these studies: collagen I (Abcam ab21286, used 1:1000 for WB, 1:250 for IF), SPARC (Cell Signaling 8725, clone D10F10, used 1:1000 for WB), GM130 (BD Biosciences 610822, clone 35/GM130, used 1:250 for IF),

(13) Slug regulates the Dll4-Notch-VEGFR2 axis to control endothelial cell activation and angiogenesisNature communicationsOctober 26, 2020Hultgren NW, Fang JS, Ziegler ME, Ramirez RN, Phan DTT, Hatch MMS, Welch-Reardon KM, Paniagua AE, Kim LS, Shon NN, et al.for western blot: mouse anti-alpha-SMA (1:1000, Dako M0851), rabbit anti-SPARC (1:1000, Cell Signaling 8725), mouse anti-CCND1 (1:1000, Santa Cruz Biotech, sc-8396), rabbit anti-p21 (1:1000, Cell Signaling 2947),

(14) In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffnessBreast cancer research : BCRMay 5, 2020Wormsbaecher C, Hindman AR, Avendano A, Cortes-Medina M, Jones CE, Bushman A, Onua L, Kovalchin CE, Murphy AR, Helber HL, et al.Vimentin (Cell Signaling, 5741), or secreted protein acidic and rich in cysteine (SPARC, Cell Signaling, 8725) for 30 min at 4 °C. Again, cells were washed with 3% BSA in PBS and then followed by incubation with

(15) Mitochondrial Fission and Mitophagy Reciprocally Orchestrate Cardiac Fibroblasts ActivationFrontiers in cell and developmental biologyJanuary 1, 2020Gao QY, Zhang HF, Tao J, Chen ZT, Liu CY, Liu WH, Wu MX, Yin WY, Gao GH, Xie Y, et al.Postn (NBP1-82472, 1:1,000; Novus Biologicals), S100A4 (AF4138, 1:1,000; Novus Biologicals), Sparc (8725S, 1:1,000; Cell Signaling Technology), Pfkfb3 (13123S, 1:1,000; Cell Signaling Technology), Pdh (3205S,

(16) Albumin tailoring fluorescence and photothermal conversion effect of near-infrared-II fluorophore with aggregation-induced emission characteristicsNature CommunicationsDecember 1, 2019Shuai Gao et al.4′,6-diamidino-2-phenylindole (DAPI) and rabbit anti-mouse SPARC (D10F10) monoclonal antibodies (1:1000, CST, USA, #8725). The secondary antibody was Alexa Fluor 555-conjugated goat anti-rabbit IgG (1:600, Invitrogen, USA,

(17) H19 lncRNA identified as a master regulator of genes that drive uterine leiomyomasOncogeneJuly 1, 2019Cao T, Jiang Y, Wang Z, Zhang N, Al-Hendy A, Mamillapalli R, Kallen AN, Kodaman P, Taylor HS, Li D, et al.dilution of 1/500), GRAF1 (Cell Signaling, 8802; used at a dilution of 1/500), SPARC (Cell Signaling, 8725; used at a dilution of 1/500), COL3A1 (LS-Bio, LS-C159386; used at a dilution of 1/1000), COL4A1 (LS-Bio,

(18) Prefrontal cortex-dependent innate behaviors are altered by selective knockdown of Gad1 in neuropeptide Y interneuronsPLOS ONEJuly 19, 2018Katelynn M. Corder, Mariana A. Cortes, Aundrea F. Bartley, Samantha A. Lear, Farah D. Lubin, Lynn E. Dobrunz

(19) Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease modelsCell researchDecember 1, 2017Yu M, Fu Y, Liang Y, Song H, Yao Y, Wu P, Yao Y, Pan Y, Wen X, Ma L, et al.

(20) Psychedelics Recruit Multiple Cellular Types and Produce Complex Transcriptional Responses Within the BrainEBioMedicineSeptember 1, 2016Martin DA, Nichols CD.

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SPARC (D10F10) Rabbit mAb

SPARC (D10F10) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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SPARC (D10F10) Rabbit mAb from Cell Signaling Technology

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