K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb 8081S from Cell Signaling Technology

Product Specs
ItemK48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb
CompanyCell Signaling Technology
Price $401.00 Inquire
Catalog Number8081S
Quantity100 µl
ApplicationsW
ReactivityAll
IsotypeIgG
CloneD9D5
HostRabbit
ClonalityMonoclonal
Research AreaUbiquitin and Ubiquitin-like proteins

Questions and Answers

Can this antibody be used to study the role of K48-linked polyubiquitination in cancer cell resistance to chemotherapy?

Yes, the K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb has been utilized in studies examining the mechanisms of chemotherapy resistance, such as in neuroblastoma cells where K48-linked polyubiquitination was shown to sequester activated caspase 3 in aggresomes, impairing apoptosis and promoting cell survival during bortezomib treatment ().

Is this antibody effective for detecting K48-linked polyubiquitin in various species?

The antibody has been shown to react with a wide range of species, including human, mouse, zebrafish, and various other mammals and non-mammalian organisms, making it suitable for comparative studies across different biological models.

How has this antibody been applied in research related to inflammatory bowel disease?

The K48-linkage Specific Polyubiquitin antibody has been used to investigate the role of deubiquitinating enzymes in inflammatory bowel disease, where it was shown that K48-linked polyubiquitination of RIPK2 is regulated by OTUB2, impacting NOD2 signaling and macrophage responses in colitis models ().

What experimental techniques can this antibody be used with?

This antibody is primarily used in Western blotting, as demonstrated in various studies where it was employed to analyze protein samples for K48-linked polyubiquitination, providing insights into protein degradation pathways and cellular responses (, ).

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Ubiquitin ligase ITCH regulates life cycle of SARS-CoV-2 virusElifeMay 29, 2026Qiwang Xiang, Camille Wouters, Peixi Chang, Yu-Ning Lu, Mingming Liu, Haocheng Wang, Haley Heine, Sunning Qian, Junqin Yang, Andrew Pekosz, et al.antibody (Enzo Life Sciences, BML-PW0600-0100); K48-linkage-specific antibody (Cell Signaling Technology, 8081S); Spike (Proteintech, 28867-1-AP); M (Proteintech, 28882-1-AP); E (Proteintech, 28904-1-AP); ITCH (Santa Cruz

(2) Inhibiting TRIM21 neddylation rejuvenates oocyte quality in PCOS by regulating ubiquitination of CPT1AResearch (Wash D C)April 3, 2026Xinni Na, Jin-Chi Liu, YINAN TAN, LINGBO MENG, CUISHAN GUO, NA ZUO, WANLIN DAI, Ruiting Cong, BOWEN ZHANG, WANTING SHI, et al.anti-ubiquitin (Cell Signaling Technology, cat# 58395), anti-ubiquitin K48 (Cell Signaling Technology, cat# 8081), anti-ubiquitin K48 (Cell Signaling Technology, cat# 5621), anti-α-tubulin (Cell Signaling Technology,

(3) The ribosome-associated N-terminal acetyltransferase B coordinates global proteostasis and autophagy in plants by creating Ac/N-degronsNature CommunicationsMarch 31, 2026Xiaodi Gong, Marlena Pożoga, Jean-Baptiste Boyer, Yuxing Xue, Thierry Meinnel, Tanja Bange, Carmela Giglione, Rüdiger Hell, Markus Wirtz(ChromoTek, Pabg1-100, 1:2,000 and Santa-Cruz Biotechnology, sc-9996 HRP, 1:2,000), K48-Ubi (Cell signaling, 8081, 1:5,000), K63-Ubi (Cell signaling, 5621, 1:1,000), Neutravidin-HRP (Invitrogen, 31001, 1:10,000), mono-

(4) OTUB2/ALYREF axis modulates the docetaxel resistance of castration-resistant prostate cancer via upregulating ABCG4-mediated drug effluxInt J Biol SciMarch 17, 2026Zhi-Bin Ke, Jia-Yin Chen, Bin Lin, Chao-Ran Chen, Yu-Ting Xue, Jiang-Bo Sun, Zi-Heng Yan, Yu-Xuan Zhao, Meng-Xin Liu, Zhen Wang, et al.60004-1-Ig), anti-polyubiquitin (1:1000; CST; 58395), anti-K48 linkage specific polyubiquitin (1:1000; CST; 8081S), anti-HA (1:1000; proteintech; 51064-2-AP), anti-Flag (1:1000; sigma; F1804), anti-His (1:1000; proteintech;

(5) Ubiquitin-Conjugating Enzyme E2O Primes Hepatocytes to Restore Immune Tolerance in Autoimmune Hepatitis via Inhibiting Y-Box Binding Protein 1/Interleukin-6 AxisCellular and Molecular Gastroenterology and HepatologyMarch 2, 2026Yu Lei, Han Wang, Yu Chen, Shuhui Wang, Zhipeng Du, Zheng Huang, Muru Wang, Shangshu Nie, Ping Han, Wei Yan, et al.cat#66008-4-Ig), Ub (Cell signaling technology, cat#20326), K48-Ub (Cell signaling technology, cat#8081), and Normal IgG (Santa Cruz, cat#sc-2025) overnight at 4°C. Then, Protein A/G Magnetic Beads (cat#HY-K0202,

(6) ISGylation prevents autophagic degradation of STING and promotes antitumor immunity in lung cancerCell Death & DiseaseFebruary 27, 2026Dan Cao, Bin Huang, Xinming Fu, Ming Liu, Xiaogang Niu, Hongbin Zhai, Hao Huang(Cell Signaling Technology, 39749), anti-Ub (Cell Signaling Technology, 20326), anti-K48-Ub (Cell Signaling Technology, 8081), anti-GAPDH (Cell Signaling Technology, 5174), anti-rabbit IgG, HRP-linked antibody (Cell Signaling Technology

(7) STI1 domain engages transient helices to mediate Dsk2 phase separation and proteasome condensationThe EMBO JournalFebruary 11, 2026Nirbhik Acharya, Emily A Daniel, Thuy P Dao, Jessica K Niblo, Erin O Mulvey, Shahar Sukenik, Daniel A Kraut, Jeroen Roelofs, Carlos A Castañeda(Invitrogen, catalog no. 459250, 1:10,000), K48-linked polyubiquitin (Cell Signaling Technology, catalog no. 8081, 1:1000), or ALFA Tag (Cell Signaling Technology, catalog no. 8081, 1:1000) and HRP-conjugated secondary

(8) K48-ubiquitin-dependent proteases cut-up post-ER proteinsNature CommunicationsJanuary 13, 2026Annabel Y. Minard, Stanley Winistorfer, Liping Yu, Robert C. PiperOregon), each at 1:1000–1:2000. K48-linked polyUb was detected using rabbit monoclonal α-K48Ub (CST; Cat. 8081; 1:1000). Secondary antibodies (α-mouse, CST #7076; α-rabbit, CST #7074S) were used at a dilution of

(9) CMTM6 promotes synovial proliferation and macrophage polarization by preventing ubiquitination of TAK1 in rheumatoid arthritisJournal of Orthopaedic TranslationJanuary 2, 2026Xiaomeng Luo, Lingling Xu, Juan Cai, Siyang Ma, Haining Song, Pingting Yang, Chunshu Yanganti-ubiquitin (P4D1) (3936, Cell Signaling Technology); rabbit anti-K48-linkage Specific Polyubiquitin (D9D5) (8081, Cell Signaling Technology); rabbit anti-CD206 (18704-1-AP, proteintech); rabbit anti-iNOS (22226-1-AP,

(10) Coupling proteostasis and de novo purine biosynthesis of PSMD14 fuels glioblastoma progression and chemoresistanceTheranosticsJanuary 1, 2026Jiazheng Wang, Qun Cao, Zhikai Li, Xuxiu Lu, Zhuo Li, Chenghui Guo, Yuan Pan, Qing Zhang, Wenjie Li, Guo Xiang, et al.Cell Signaling Technology), ubiquitin (20326S; 1:1,000; Cell Signaling Technology), K48-linkage-specific polyubiquitin (8081S; 1:1,000; Cell Signaling Technology), K63-linkage-specific polyubiquitin (5621S; 1:1,000; Cell Signaling Technology

(11) TRIM47-facilitated PLK1 stabilization promotes the proliferation of liver cancer cellsCell Oncol (Dordr)December 29, 2025Tao Xie, Xiaoqi Fan, Tian Yu, Qing Zhu, Qi Zhang, Na Li, Tao Wang, Yisong Qian, Keyu Deng, Hongbo Xin, et al.p-p38 (4511S), Akt (9272S), p-Akt (4060S), K63-linked specific Ub (5621S), K48-linked specific Ub (8081S), and Ub (3936S) were purchased from Cell Signaling Technology (Danvers, MA, USA). TRIM47 (H00091107-M02)

(12) Deubiquitinase MoAMSH inhibits autophagy by targeting MoAtg6 in Magnaporthe oryzaeCell Communication and SignalingNovember 27, 2025Jian Liao, Zi-Fang Shen, Jing-Yi Wang, Meng-Fan He, Hong-Min Lv, Jin Zhao, Yun‑Ran Zhang, Xue‑Ming Zhu, Lin Li, Zi‑He Wang, et al.(HUABIO; ET1703-07; 1:1000), and Anti-K48-linkage specific ubiquitin antibody (Cell Signaling Technology; 8081; 1:1000) was used to detect ubiquitinated, K63-linkage specific ubiquitinated, and K48-linkage specific

(13) Treatment with L-type amino acid transporter 1 inhibitor JPH203 enhances protein synthesis in C2C12 myotubesScientific ReportsNovember 19, 2025Junya Takegaki, Takaoki Saneyasu, Kazuhisa Honda(1:1000, sc-398608, Santa Cruz Biotechnology, TX, USA), K48 linkage-specific polyubiquitin (1:1000, #8081, CST), phospho-ULK1 (1:1000. Ser757, #14202, CST; 1:1000, Ser555, #5869, CST), total-ULK1 (1:1000, #8054,

(14) Palmitoylation of TBK1 enhances the type I interferon signaling and strengthens anti-malarial immunity in miceNature CommunicationsNovember 18, 2025Zhongxin Han, Siyi Xiong, Ke Zeng, Zilong Xiao, Liying Zhang, Yufen Zhang, Jiaying Guo, Wenqiang Peng, Yingchao Xie, Weiwei Liu, et al.(Cat#2276, 1:1000, clone name 9B11), anti-p62 (Cat#88588, 1:1000, clone name D5L7G), anti-K48-Ub (Cat#8081, 1:1000, clone name D9D5), anti-phosphor-STING (Ser365) (Cat#72971, 1:1000, clone name D8F4W) were purchased

(15) ITCH regulates Golgi integrity and proteotoxicity in neurodegenerationSci AdvOctober 24, 2025Qiwang Xiang, Yuning Lu, Hongjin Wang, Hao Chen, Peng Chen, Xiaofeng Zhao, Larissa Cortes Morales, Yiwei Yang, Junqin Yang, Tao Zhang, et al.(Cell Signaling Technology, 58395S, 1:1000 for WB); K48-linkage–specific antibody (Cell Signaling Technology, 8081S, 1:1000 for WB); ITCH (Santa Cruz, sc-28367, 1:1000 for WB, 1:50 for IF); ITCH (Novus Biologicals, NB100-68142,

(16) TRIM45 restricts influenza virus infection through modulating the chaperone-mediated autophagic degradation of viral PB2 proteinPLoS PathogOctober 23, 2025Yihan Wang, Li Jiang, Qibing Li, Mengya Li, Wenjun Shi, Bo Wang, Guangwen Wang, Guohua Deng, Jianzhong Shi, Guobin Tian, et al.13750–1-AP; 1:1000), rabbit anti-K48-linkage specific polyubiquitin mAb (Cell Signaling Technology, 8081S; 1:1000), rabbit anti-K63-linkage specific polyubiquitin mAb (Cell Signaling Technology, 5621S; 1:1000),

(17) Deacetylation of TALDO1 by HDAC6 promotes glycolysis and nasopharyngeal carcinoma progression through a moonlighting functionCell Death DisOctober 21, 2025Xingzhi Peng, Peijun Zhou, Kun Zhang, Likang Chen, Min Tang, Qin Zhou, Jinwu Peng, Lifang YangSanta Cruz Biotechnology (Santa Cruz, CA); Actin (AC026) from Abclonal (Wuhan, China). K63-Ub (#5621), K48-Ub (#8081), PDK1 (#3062), LDHA (#3582), PKM2 (#4053), and Histone H3 (#4499) from Cell Signaling Technology (Danvers,

(18) Targeting Intratumoral Copper Inhibits Tumor Progression via p62-Mediated EZH2 Degradation and Potentiates Anti-PD-1 Immunotherapy in Oral Squamous Cell CarcinomaAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2025Lin X, Chen W, Li B, Zhao Z, Yu Z, Zhao XY, Zhou X, Feng Z, Lin C, Cao W.(A19056, Abclonal, China, dilution 1:1000, RRID: AB_2 862 549), K48‐linkage Specific Polyubiquitin(#8081, CST, USA, dilution 1:1000, RRID: AB_10 859 893), K63‐linkage Specific Polyubiquitin(#5621, CST, USA,

(19) Polyubiquitin architecture editing on collided ribosomes maintains persistent RQC activityThe EMBO JournalSeptember 16, 2025Shota Tomomatsu, Yoshitaka Matsuo, Fumiaki Ohtake, Takuya Tomita, Yasushi Saeki, Toshifumi InadaSanta Cruz Biotechnology (# sc-0817, RRID:AB_628423); Anti-K48 Ubiquitin antibody from Cell Signaling Technology (# 8081; RRID:AB_1587580); Anti-K63 Ubiquitin antibody from EMD Millipore (# 05-1308; RRID:AB_10859893); ECL

(20) Targeting Methylglyoxal Metabolism to Enhance Ferroptosis Sensitivity in Tumor TherapyAdvanced ScienceSeptember 4, 2025Xinyue Zhang, Leng Han, Zimu Wang, Hanghui Yu, Jiao Liu, Rui Kang, Daolin Tang, Zhengjia Liu, Xianlong Du, Enyong Dai(4393), AIFM2 (24972), SLC7A11 (12691), AMPK (2532), p‐AMPK (2535), K48‐linkage‐specific polyubiquitin (8081), K63‐linkage‐specific polyubiquitin (5621), CD8 (98941), CD4 (25229), SQSTM1 (5114), and ACTB (4970)

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K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb

K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb from Cell Signaling Technology

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