Fig 1: Photomicrographs of the differential expression of pro-AGMs (PIGF and VEGF-A), anti-AGMs (Eng and Flt-1), and oxidative DNA marker (8-OHdG) in placentae from SHS and OHS-PWD EO-PE and LO-PE compared to NTN-P.Negative control showing negative or no IHC staining (column 1). Higher staining intensity/diffuse positivity (high brown staining) of expression of Eng (row 1), Flt-1 (row 2) in cytotrophoblast, syncytiotrophoblast, mesenchymal cells of the villous stroma and syncytial knots of SHS- pregnant women who developed EO-PE at 32 weeks as compared to OHS- pregnant women who developed EO-PE at 32 weeks gestation. Partial positivity (moderate brown staining) expression in SHS- pregnant women who developed LO-PE at 37 weeks, focal positivity (low brown staining) expression in OHS- pregnant women who developed LO-PE at 37 weeks and negative expression in NTN-pregnant women at 38 weeks. Higher staining intensity/diffuse positivity (high brown staining) of expression of VEGF (row 3) and PlGF (row 4) in endothelial cells of foetal capillaries and syncytiotrophoblast of NTN-pregnant women at 38 weeks. Focal positivity (low brown staining) expression in SHS and OHS- pregnant women who developed LO-PE at 37 weeks and negative expression in SHS and OHS- pregnant women who developed EO-PE at 32 weeks. Higher staining intensity/diffuse positivity (high brown staining) of expression of 8-OHdG (row 5) in cytotrophoblast cell nuclei and syncytiotrophoblast of SHS- pregnant women who developed EO-PE and OHS- pregnant women who developed EO-PE at 32 weeks gestation. Partial positivity (moderate brown staining) expression in SHS- pregnant women who developed LO-PE at 37 weeks, focal positivity (low brown staining)/negative expression in OHS- pregnant women who developed LO-PE at 37 weeks and NTN-pregnant women at 38 weeks (magnification x20). Scale bar = 200μm.