Galectin-9 Antibody AF3535 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGalectin-9 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF3535
ApplicationsFlow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Intracellular Staining by Flow Cytometry,Immunocytochemistry,CyTOF-ready,ELISA Capture
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityMouse,Rat
Quantity100 ug (also 25 ug)
TargetGalectin-9
IsotypeIgG
Conjugate/TagUnconjugated
HostGoat
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse Galectin-9, Ala2-Thr353; aa 148-178 deletion, Accession # O08573
ConcentrationLYOPH
PurityAntigen Affinity-purified
NCBI Full Gene Namelectin, galactose binding, soluble 9, galectin 9
NCBI Gene AliasesHUAT, galectin-9, LGALS35, gal-9, LGALS9A, Lgals5
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Galectin-9 in direct ELISAs and Western blots. In Western blots, approximately 30% cross-reactivity with recombinant human (rh) Galectin-9 is observed. In direct ELISAs and Western blots, less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-3, rhGalectin-7, rhGalectin-8, recombinant mouse (rm) Galectin-1, rmGalectin-3, rmGalectin-4, and rmGalectin-7 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Spatially resolved transcriptomics identifies intercellular signaling post-ischemic stroke that controls neural stem cell proliferationStem Cell ReportsJune 9, 2026He Huang, Emerson Daniele, Wing Chung Jessie Lam, Teodora Tockovska, Daniela Lozano Casasbuenas, Hathairat Chanphao, Bebhinn Treanor, Maryam Faiz, Scott A. Yuzwafor immunohistochemistry (IHC) in this study included: goat anti-mouse galectin-9 (R&D Systems, Cat#AF3535, RRID: AB_2137240) reconstituted at 0.2 mg/mL in sterile PBS and diluted at 1:30; rabbit anti-doublecortin (DCX

(2) Gangliosides modulate insulin secretion by pancreatic beta cells under glucose stressGlycobiologyAugust 20, 2020Jennemann R, Kaden S, Volz M, Nordström V, Herzer S, Sandhoff R, Gröne HJ.PBS 0.05% Tween 20, followed by a second incubation step with goat anti-mouse galectin-9 antibodies (AF3535, R&D Systems), 1:1000 diluted in 1% BSA in PBS at room temperature for 4 h. The plates were washed and

(3) N-Linked Glycosylation Regulates CD22 Organization and FunctionFrontiers in immunologyJanuary 1, 2019Wasim L, Buhari FHM, Yoganathan M, Sicard T, Ereño-Orbea J, Julien JP, Treanor B.Cells were washed two times and immunostained with goat anti-galectin-9 antibody (R&D Systems, Cat. No. AF3535) at 1:100 v/v for 1 h in 2% BSA in PBS. Cells were washed 3 times, followed by labeling with Cy3-conjugated

(4) Galectin-9 binds IgM-BCR to regulate B cell signalingNature communicationsAugust 17, 2018Cao A, Alluqmani N, Buhari FHM, Wasim L, Smith LK, Quaile AT, Shannon M, Hakim Z, Furmli H, Owen DM, et al.B220-allophycocyanin (APC) (clone RA3-6B2, Invitrogen), and goat anti-mouse galectin-9 (R&D Systems, Cat. No. AF3535) at 1:200 v/v in blocking buffer for 1 h at room temperature. Sections were washed five times with 0.5%

(5) Galectin-9 Is Critical for Mucosal Adaptive Immunity through the T Helper 17-IgA AxisThe American journal of pathologyMay 1, 2018Liang CC, Li CS, Weng IC, Chen HY, Lu HH, Huang CC, Liu FT.

(6) Characterization of neutralizing antibodies reacting with the 213-224 amino-acid segment of human galectin-9PloS oneJanuary 1, 2018Lhuillier C, Barjon C, Baloche V, Niki T, Gelin A, Mustapha R, Claër L, Hoos S, Chiba Y, Ueno M, et al.

(7) Astrocyte galectin-9 potentiates microglial TNF secretionJournal of neuroinflammationAugust 27, 2014Steelman AJ, Li J.

(8) Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosisThe Journal of biological chemistryAugust 16, 2013Steelman AJ, Smith R 3rd, Welsh CJ, Li J.

(9) TIM-3 does not act as a receptor for galectin-9PLoS pathogensMarch 1, 2013Leitner J, Rieger A, Pickl WF, Zlabinger G, Grabmeier-Pfistershammer K, Steinberger P.

(10) Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effectThe American journal of pathologyMarch 1, 2011Iqbal AJ, Sampaio AL, Maione F, Greco KV, Niki T, Hirashima M, Perretti M, Cooper D.

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(1)

7/3/2020Antibody Did Not Work as Expected for the Western Blots Shubhankar AmbikeI would not recommend this product for WB analysis from macrophage cell line. It did not work with cell lysate and cell supernatant. In general, for analysis of Gal-9 expression levels, please opt for... Read Review

Galectin-9 Antibody

Galectin-9 Antibody

Galectin-9 Antibody

Galectin-9 Antibody

Galectin-9 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (10)

Reviews

Galectin-9 Antibody from R&D Systems, a Bio-Techne Brand

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