FoxO1 (Ser256) Polyclonal Antibody bs-3142R from Bioss Inc.

Product Specs
ItemFoxO1 (Ser256) Polyclonal Antibody
CompanyBioss Inc.
Price $375.00Supplier Page View Company Product Page
Catalog Numberbs-3142R
Molecular Weight72kDa
ImmunogenKLH conjugated phosphopeptide, human FOXO1 around Ser256 [AA(p-S)MD]
IsotypeIgG
ApplicationsWB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), FCM, WB
Conjugate/TagUnconjugated
Format0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol
ReactivityHuman, Mouse, Rat, Rabbit, Other Species, Human, Bovine
Quantity100ul, 20uL
ClonalityPolyclonal
Antibody TypePrimary Antibody
HostRabbit
TargetFoxO1 Ser256
Concentration1ug/ul
NCBI Gene AliasesFKH1, FKHR, FOXO1A
NCBI Full Gene Nameforkhead box O1
ModificationsSer256

Questions and Answers

Can this antibody be used to study the phosphorylation status of FoxO1 in dairy cows?

Yes, the FoxO1 (Ser256) Polyclonal Antibody has been utilized in studies examining the phosphorylation of FoxO1 at serine 256 in dairy cows. For instance, in a study by Kinoshita et al. (2016), the antibody was employed in Western blotting to analyze the extent of phosphorylation of FoxO1 in hepatic tissue, demonstrating its applicability in this context.

What species can I use this antibody with in my experiments?

This antibody is reactive with multiple species, including humans, mice, rats, and bovines. This broad reactivity makes it suitable for various applications across different biological models.

Is there any evidence of this antibody's effectiveness in detecting FoxO1 protein levels?

The FoxO1 (Ser256) Polyclonal Antibody has been shown to effectively detect phosphorylated FoxO1 protein levels in Western blot analyses, as indicated in the study by Kinoshita et al. (2016). The antibody was used at a dilution of 1:200 for detecting phosphorylated FoxO1, confirming its utility in protein expression studies.

How does the phosphorylation of FoxO1 at serine 256 affect its function?

Phosphorylation of FoxO1 at serine 256 is known to inhibit its activity, which is relevant in the context of insulin signaling and hepatic glucose production. The antibody can be instrumental in studying these regulatory mechanisms, as demonstrated in the research involving dairy cows, where the relationship between FoxO1 phosphorylation and glucose production was explored.

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(1) PD-1 induces autophagy via the PI3K/AKT/FoxO1 pathway to promote infectious bursal disease virus replicationFront ImmunolJuly 7, 2025Qiuyu Zhang, Feng Yue, Guopeng Sun, Liwei Jiang, Peng Li, Yanping Zhu, Zhike Liu, Yangzhao Zhu, Ruiyan Niu, Hua He, et al.purchased from Abmart (Shanghai, China). Anti-FoxO1 (1:1000; cat# bs-9439R and anti-p-FoxO1 (1:1000; cat# bs-3142R) antibodies were purchased from Bioss (Beijing, China). Anti-chicken PD-1 (1:100) mouse monoclonal antibodies

(2) TIGIT signaling restores suppressor function of Th1 TregsJCI insightFebruary 7, 2019Lucca LE, Axisa PP, Singer ER, Nolan NM, Dominguez-Villar M, Hafler DA.

(3) Long-term thermal manipulation in the late incubation period can inhibit breast muscle development by activating endoplasmic reticulum stress in duck (Anasplatyrhynchos domestica)Journal of thermal biologyDecember 1, 2017Li X, Qiu J, Liu H, Wang Y, Hu J, Gan X, Wang J.

(4) Associations between Forkhead Box O1 (FoxO1) Expression and Indicators of Hepatic Glucose Production in Transition Dairy Cows Supplemented with Dietary Nicotinic AcidPloS oneJanuary 1, 2016Kinoshita A, Locher L, Tienken R, Meyer U, Dänicke S, Rehage J, Huber K.phosphorylated FoxO1 protein at serine 256 (pFoxO1) (rabbit anti-FoxO1 (Ser256) polyclonal antibody, bs-3142R, Bioss Inc.), as well as 1:10000 for beta-actin (mouse anti-beta-actin monoclonal antibody, A5441, Sigma-Aldrich,

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FoxO1 (Ser256) Polyclonal Antibody

FoxO1 (Ser256) Polyclonal Antibody from Bioss Inc.

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Questions and Answers

Bioss Inc.

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FoxO1 (Ser256) Polyclonal Antibody from Bioss Inc.

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