ChAT Polyclonal Antibody bs-0042R from Bioss Inc.

Product Specs
ItemChAT Polyclonal Antibody
CompanyBioss Inc.
Price $375.00Supplier Page View Company Product Page
Catalog Numberbs-0042R
Molecular Weight68-70kDa
NCBI Gene AliasesCMS1A, CMS1A2, CHOACTASE, CMS6
ImmunogenKLH conjugated synthetic peptide derived from human ChAT
Conjugate/TagUnconjugated
ReactivityMouse, Dog, Human
Antibody TypePrimary Antibody
Quantity100ul, 20uL
Format0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol
IsotypeIgG
ApplicationsIHC-P, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
ClonalityPolyclonal
HostRabbit
TargetChAT
Concentration1ug/ul
NCBI Full Gene Namecholine O-acetyltransferase
Tissuebrain

Questions and Answers

What species does this antibody work with, and has it been used in studies involving those species?

The ChAT Polyclonal Antibody is reactive with Canine, Mouse, Human, and Rat tissues. It has been cited in six scientific articles, indicating its utility in various studies involving these species, particularly in the context of neuroscience research.

Can this antibody be used for immunohistochemistry, and what kind of results can I expect?

Yes, the ChAT Polyclonal Antibody is suitable for immunohistochemistry (IHC) applications, both in frozen (IHC-fr) and paraffin-embedded (IHC-p) tissues. Users can expect reliable staining of cholinergic neurons, which is crucial for studying acetylcholine synthesis and related neurological functions.

Is this antibody effective for flow cytometry, and are there any specific protocols or conditions recommended?

The ChAT Polyclonal Antibody has been shown to work in flow cytometry (FCM). While specific protocols are not provided, it is generally recommended to optimize antibody concentration and incubation times based on the specific cell types and experimental conditions being used.

What is the immunogen for this antibody, and how does it relate to its specificity?

The immunogen for the ChAT Polyclonal Antibody is a KLH-conjugated synthetic peptide derived from human ChAT. This design enhances the antibody's specificity for the ChAT protein, which is essential for accurately detecting acetylcholine synthesis in various experimental settings.

Bioss Inc.

300 Tradecenter Drive

Suite 4610

Woburn, MA 01801

United States

Phone: 800.501.7654 (Toll free)
Phone: +1.781.569.5821 (International)

Email: info@biossusa.com
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Company Profile

Website: www.biossusa.com

(1) Epineural stimulation on distal brachial plexus for functional restoration of the upper limb in a primate studyFrontiers in NeurologyMarch 18, 2025Tianfang Yan, Benjamin C. Fortune, Lingjun Liu, Yan Liu, Taro Kaiju, Takafumi Suzuki, Masayuki Hiratamin at room temperature, the tissues were incubated with primary rabbit anti-ChAT antibody (1:250, bs-0042R, Bioss, United States) overnight at 4°C. After washing with phosphate buffer saline (0.05 M PBS, pH

(2) Effects of targeted muscle reinnervation on spinal cord motor neurons in rats following tibial nerve transectionNeural Regeneration ResearchJanuary 12, 2022Lin Yang, Xue-Zheng Liu, Zhen-Dong Jiang, Jian-Ping Yang, Zhen-Dong JiangRRID:AB_2895151, Bioss, Woburn, MA, USA) or rabbit anti-ChAT antibody to stain spinal cords (1:400, Cat# bs-0042R, RRID: AB_10856836, Bioss). Sections were then incubated with horseradish peroxidase–conjugated rabbit

(3) Induced pluripotent stem cell-derived enteric neural crest cells repopulate human aganglionic tissue-engineered intestine to form key components of the enteric nervous systemJournal of tissue engineeringJanuary 1, 2020Chang DF, Zuber SM, Gilliam EA, Nucho LA, Levin G, Wang F, Squillaro AI, Huang S, Spence JR, Grikscheit TC.(Calbindin),Primary,Rabbit,1:200,Millipore #AB1776 CHAT (Choline Acetyltransferase),Primary,Rabbit,1:200,Bioss #BS-0042R CHGA (Chromogranin A),Primary,Rabbit,1:200,Abcam #ab15160 CKit (c-Kit),Primary,Rabbit,1:200,Abcam

(4) Stimulation of Epicardial Sympathetic Nerves at Different Sites Induces Cardiac Electrical Instability to Various DegreesScientific reportsJanuary 17, 2018Wang L, Sun L, Wang K, Jin Y, Liu Q, Xia Z, Liu X, Zhang J, Li J.

(5) Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is AganglionicTissue engineering. Part AJanuary 1, 2016Wieck MM, El-Nachef WN, Hou X, Spurrier RG, Holoyda KA, Schall KA, Mojica SG, Collins MK, Trecartin A, Cheng Z, et al.

(6) MicroRNA profiling of atrial fibrillation in canines: miR-206 modulates intrinsic cardiac autonomic nerve remodeling by regulating SOD1PloS oneJanuary 1, 2015Zhang Y, Zheng S, Geng Y, Xue J, Wang Z, Xie X, Wang J, Zhang S, Hou Y.

(7) Catalpol improves cholinergic function and reduces inflammatory cytokines in the senescent mice induced by D-galactoseFood and chemical toxicology : an international journal published for the British Industrial Biological Research AssociationAugust 1, 2013Zhang X, Jin C, Li Y, Guan S, Han F, Zhang S.

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ChAT Polyclonal Antibody

ChAT Polyclonal Antibody from Bioss Inc.

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Questions and Answers

Bioss Inc.

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ChAT Polyclonal Antibody from Bioss Inc.

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