Fig 2: IHC analysis of the testis tissues. Immunopositive expression of Nrf2, Keap1, SOD2, SOD3, CDC25C, and CDK1 was observed. Original magnification, ×200.

Fig 3: The mean density of six antibodies. (A). Nrf2; (B). Keap1; (C). SOD2; (D). SOD3; (E). CDC25C; (F). CDK1; Means ± SD; n = 6; *p < .05, compared with the model group; **p < .01, compared with the model group; ## p < .05, compared with the control group.

Fig 4: DQ alleviates cellular senescence, DNA damage, fibrosis, and regulates cellular apoptosis in CY-induced POI mice (n=3). A) Representative images of Ki-67, p16, p21, p53, and y-H2AX expression in murine ovaries obtained by IHC, and SA-P-gal staining images of murine ovarian sections. B) Representative images of IHC staining (8-OHdG, SOD2, and HO1), TUNEL staining, and Sirius Red-stained ovaries. All values were displayed as mean ±SD; scale bars: 100 um. One-way ANOVA was used for statistical analysis; *p <0.05, **p <0.01, ***p 0.001; ****p <0.0001.